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Viruses 2018, 10(11), 616; https://doi.org/10.3390/v10110616

Characterization of the Escherichia coli Virulent Myophage ST32

1
College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 101408, China
2
Département de Biochimie, de Microbiologie, et de Bio-Informatique, Faculté des Sciences et de Génie, Université Laval, Québec City, QC G1V 0A6, Canada
3
Félix d’Hérelle Reference Center for Bacterial Viruses, Faculté de médecine dentaire, Université Laval, Québec City, QC G1V 0A6, Canada
Current address: SyntBioLab Inc., Lévis, QC G6W 0L9, Canada.
*
Authors to whom correspondence should be addressed.
Received: 15 October 2018 / Revised: 4 November 2018 / Accepted: 6 November 2018 / Published: 7 November 2018
(This article belongs to the Special Issue Bacteriophage Genomes and Genomics: News from the Wild)
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Abstract

The virulent phage ST32 that infects the Escherichia coli strain ST130 was isolated from a wastewater sample in China and analyzed. Morphological observations showed that phage ST32 belongs to the Myoviridae family, as it has an icosahedral capsid and long contractile tail. Host range analysis showed that it exhibits a broad range of hosts including non-pathogenic and pathogenic E. coli strains. Interestingly, phage ST32 had a much larger burst size when amplified at 20 °C as compared to 30 °C or 37 °C. Its double-stranded DNA genome was sequenced and found to contain 53,092 bp with a GC content of 44.14%. Seventy-nine open reading frames (ORFs) were identified and annotated as well as a tRNA-Arg. Only nineteen ORFs were assigned putative functions. A phylogenetic tree using the large terminase subunit revealed a close relatedness with four unclassified Myoviridae phages. A comparative genomic analysis of these phages showed that the Enterobacteria phage phiEcoM-GJ1 is the closest relative to ST32 and shares the same new branch in the phylogenetic tree. Still, these two phages share only 47 of 79 ORFs with more than 90% identity. Phage ST32 has unique characteristics that make it a potential biological control agent under specific conditions. View Full-Text
Keywords: Escherichia coli; virulent phage; genomic characterization; lytic activity; biocontrol Escherichia coli; virulent phage; genomic characterization; lytic activity; biocontrol
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Liu, H.; Geagea, H.; Rousseau, G.M.; Labrie, S.J.; Tremblay, D.M.; Liu, X.; Moineau, S. Characterization of the Escherichia coli Virulent Myophage ST32. Viruses 2018, 10, 616.

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