Next Article in Journal
Mason-Pfizer Monkey Virus Envelope Glycoprotein Cycling and Its Vesicular Co-Transport with Immature Particles
Next Article in Special Issue
Autographa Californica Multiple Nucleopolyhedrovirus Enters Host Cells via Clathrin-Mediated Endocytosis and Direct Fusion with the Plasma Membrane
Previous Article in Journal
βTrCP is Required for HIV-1 Vpu Modulation of CD4, GaLV Env, and BST-2/Tetherin
Previous Article in Special Issue
Disruption of Autographa Californica Multiple Nucleopolyhedrovirus ac111 Results in Reduced per os Infectivity in a Host-Dependent Manner
Article Menu
Issue 10 (October) cover image

Export Article

Open AccessArticle
Viruses 2018, 10(10), 574;

Improved Baculovirus Vectors for Transduction and Gene Expression in Human Pancreatic Islet Cells

Department of Biological and Medical Sciences, Oxford Brookes University, Oxford OX3 0BP, UK
Oxford Expression Technologies Ltd., Bioinnovation Hub, Gipsy Lane Campus, Oxford OX3 0BP, UK
Department of Biotechnology, College of Sciences, Baghdad University, Baghdad 10071, Iraq
Centre for Molecular and Cell-Based Therapeutics SA de CV, Mexico City 15820, Mexico
Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX3 9DU, UK
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 5 October 2018 / Accepted: 18 October 2018 / Published: 20 October 2018
(This article belongs to the Special Issue Baculovirus Advances and Applications)
Full-Text   |   PDF [3042 KB, uploaded 20 October 2018]   |  


Pancreatic islet transplantation is a promising treatment for type 1 diabetes mellitus offering improved glycaemic control by restoring insulin production. Improved human pancreatic islet isolation has led to higher islet transplantation success. However, as many as 50% of islets are lost after transplantation due to immune responses and cellular injury, gene therapy presents a novel strategy to protect pancreatic islets for improved survival post-transplantation. To date, most of the vectors used in clinical trials and gene therapy studies have been derived from mammalian viruses such as adeno-associated or retrovirus. However, baculovirus BacMam vectors provide an attractive and safe alternative. Here, a novel BacMam was constructed containing a frameshift mutation within fp25, which results in virus stocks with higher infectious titres. This improved in vitro transduction when compared to control BacMams. Additionally, incorporating a truncated vesicular stomatitis virus G protein increased transduction efficacy and production of EGFP and BCL2 in human kidney (HK-2) and pancreatic islet β cells (EndoC βH3). Lastly, we have shown that our optimized BacMam vector can deliver and express egfp in intact pancreatic islet cells from human cadaveric donors. These results confirm that BacMam vectors are a viable choice for providing delivery of transgenes to pancreatic islet cells. View Full-Text
Keywords: BacMam; baculovirus; gene therapy; high-titre virus; human pancreatic islet cells BacMam; baculovirus; gene therapy; high-titre virus; human pancreatic islet cells

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Graves, L.P.; Aksular, M.; Alakeely, R.A.; Ruiz Buck, D.; Chambers, A.C.; Murguia-Meca, F.; Plata-Muñoz, J.-J.; Hughes, S.; Johnson, P.R.V.; Possee, R.D.; King, L.A. Improved Baculovirus Vectors for Transduction and Gene Expression in Human Pancreatic Islet Cells. Viruses 2018, 10, 574.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top