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Article

Enhanced Production of Recombinant Protein by Fusion Expression with Ssp DnaB Mini-Intein in the Baculovirus Expression System

1
Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University, Cheongju 28644, Korea
2
Optipharm Inc., Osong 28158, Korea
3
Diagnostic Development 2 Team, ImmuneMed Inc., Chuncheon 24232, Korea
*
Authors to whom correspondence should be addressed.
Academic Editors: Linda King and Robert Possee
Viruses 2018, 10(10), 523; https://doi.org/10.3390/v10100523
Received: 31 July 2018 / Revised: 19 September 2018 / Accepted: 24 September 2018 / Published: 25 September 2018
(This article belongs to the Special Issue Baculovirus Advances and Applications)
The baculovirus expression system (BES) is considered to be a very powerful tool for the expression of numerous difficult to express vertebrate proteins. Ssp DnaB mini-intein is a useful fusion partner for the production of recombinant proteins because it can be self-cleaved by controlling the pH and temperature, without additional treatment. To evaluate the utility of Ssp DnaB mini-intein in the BES, recombinant viruses were generated to express the enhanced green fluorescent protein, the VP2 protein of porcine parvovirus, and the E2 protein of classical swine fever virus fused to a mini-intein. As expected, intracellular self-cleavage of the mini-intein occurred during virus infection, but the cleavage initiation time varied depending on the target protein. Significantly enhanced protein production was observed for all of the target proteins that were fused to the mini-intein. This increase was enough to overcome the decrease in the fusion protein due to intracellular self-cleavage. The mini-intein in all of the recombinant fusion proteins was successfully cleaved by controlling the pH and temperature. These results suggest that the Ssp DnaB mini-intein is a useful fusion partner in the BES for easy purification and enhanced production of target proteins. View Full-Text
Keywords: baculovirus; Ssp DnaB mini-intein; fusion partner; purification; enhanced production baculovirus; Ssp DnaB mini-intein; fusion partner; purification; enhanced production
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MDPI and ACS Style

Gwak, W.S.; Choi, J.B.; Han, B.K.; Bae, S.M.; Woo, S.D. Enhanced Production of Recombinant Protein by Fusion Expression with Ssp DnaB Mini-Intein in the Baculovirus Expression System. Viruses 2018, 10, 523. https://doi.org/10.3390/v10100523

AMA Style

Gwak WS, Choi JB, Han BK, Bae SM, Woo SD. Enhanced Production of Recombinant Protein by Fusion Expression with Ssp DnaB Mini-Intein in the Baculovirus Expression System. Viruses. 2018; 10(10):523. https://doi.org/10.3390/v10100523

Chicago/Turabian Style

Gwak, Won S.; Choi, Jae B.; Han, Beom K.; Bae, Sung M.; Woo, Soo D. 2018. "Enhanced Production of Recombinant Protein by Fusion Expression with Ssp DnaB Mini-Intein in the Baculovirus Expression System" Viruses 10, no. 10: 523. https://doi.org/10.3390/v10100523

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Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

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