Characterization of CiWRI1 from Carya illinoinensis in Seed Oil Biosynthesis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Growth Conditions
2.2. Gene Cloning
2.3. Plasmid Construction
2.4. Total RNA Extraction and Quantitative Reverse Transcription (qRT)-PCR
2.5. Recombinant CiWRI1 Protein Expression and Electrophoretic Mobility Shift Assay (EMSA)
2.6. Nicotiana Benthamiana Infiltration
2.7. Lipid Analysis
2.8. Statistical Analysis
3. Results
3.1. Energy Metabolism in Two Varieties of Pecan with Different Mature Periods
3.2. Expression Patterns of Lipid Synthesis Genes in Pecan
3.3. Structural Features of C. Illinoinensis WRI1
3.4. CiWRI1 is Functional Conserved with WRI1 in Arabidopsis
3.5. Cloning of the ACCase Subunit BCCP2
3.6. Regulation of CiBCCP2 Expression by CiWRI1
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Variety | Name | Maturation Time(DAF) | Seed Oil Content (%) | Soluble Sugar Content (%) | Protein Content (mg/g) | Starch Content (mg/g) |
---|---|---|---|---|---|---|
Early maturing | Pawnee | 152 ± 1.41 | 74.43 ± 1.32 | 1.68 ± 0.01 | 2.11 ± 0.16 | 17.76 ± 4.36 |
Late maturing | Mahan | 173 ± 0.82 *** | 71.59 ± 0.79 * | 1.75 ± 0.01 | 3.85 ± 0.54 * | 7.09 ± 2.18 ** |
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Zhou, X.; Dai, Y.; Wu, H.; Zhong, P.; Luo, L.; Shang, Y.; Tan, P.; Peng, F.; Tian, Z. Characterization of CiWRI1 from Carya illinoinensis in Seed Oil Biosynthesis. Forests 2020, 11, 818. https://doi.org/10.3390/f11080818
Zhou X, Dai Y, Wu H, Zhong P, Luo L, Shang Y, Tan P, Peng F, Tian Z. Characterization of CiWRI1 from Carya illinoinensis in Seed Oil Biosynthesis. Forests. 2020; 11(8):818. https://doi.org/10.3390/f11080818
Chicago/Turabian StyleZhou, Xiaofeng, Yuqiu Dai, Haijun Wu, Peiqiao Zhong, Linjie Luo, Yangjuan Shang, Pengpeng Tan, Fangren Peng, and Zhaoxia Tian. 2020. "Characterization of CiWRI1 from Carya illinoinensis in Seed Oil Biosynthesis" Forests 11, no. 8: 818. https://doi.org/10.3390/f11080818