Characterization of CiWRI1 from Carya illinoinensis in Seed Oil Biosynthesis
, Yuqiu Dai 2, Haijun Wu 2, Peiqiao Zhong 3, Linjie Luo 4, Yangjuan Shang 1, Pengpeng Tan 1, Fangren Peng 1,* and Zhaoxia Tian 2,*Round 1
Reviewer 1 Report
The topic of the article is interesting and significant. Scientific soundness of the manuscript is high. However, the manuscript needs the revision.
Comment 1: The introduction was written very well. In this work, I lacked information on the weather conditions during cultivation Pawnee and Mahan. Of course, the effect of the weather on the results obtained should also be described.
Comment 2: Were both genotypes growing in the same conditions and in the same year? When and where was the field experiment?
Comment 3: Line 133-141. There is no information about the sample size for analysis, the number of repetitions.
Comment 4: Results: lines 158-163 Such general conclusions should not be drawn when the authors analyze only two varieties and only in one (?) year of research
Comment 5: Please analyze the described results (158-168) in relation to weather conditions
Comment 6: The authors write: “Given the higher oil and starch contents in the Pawnee variety of pecan (Figure 1a)”. What I see in Figure 1: the differences in oil content between varieties are significant in the range of 122-132 DAF. At 152 DAF, no significant difference was observed. Therefore, the above sentence is not true.
Comment 7: Lines 192-200 Have the authors checked the impact of environmental conditions on gene expression in other species?
Comment 8: The authors refer all obtained results to the maturation date of varieties. Has this relationship been seen in other species? Or is it only due to the genotype?
Comment 9: The molecular part is written well
Comment 10: The discussion lacks a broader comparison with results on other oilseeds
Author Response
Please see the attachment.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
In the manuscript ‘Characterization of CiWRI1 from Carya illinoinensis in seed oil biosynthesis’, the authors present their work on WRI1 from Carya illinoinensis performed in the same manner as in the AtWRI1. The authors executed the qRT-PCR analysis, the complementation test, the cellular localization assay and the EMSA assay.
This work provides an important contribution to understanding of seed oil metabolism in Carya illinoinensis.
I have comments:
1. The authors indicated that seed oil content was partially rescued by the introduction of 35S::CiWRI1 into wri1-1 in Fig. 4e. Detailed explanation is necessary on this point. There are several reports that 35S promotor was less effective in Arabidopsis seeds. These papers will be references.
2. Researches in the plant oil biosynthesis are of particular interest in the differences of the DNA sequence between WRI1 (from promoter to terminator) in Pawnee and that in Mahan. Addition of the sequence data will enhance the importance of this study.
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
Round 2
Reviewer 1 Report
Thank you for making corrections to the manuscript.I have no more objections.
