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Identification the Pathogens Causing Rot Disease in Pomegranate (Punica granatum L.) in China and the Antifungal Activity of Aqueous Garlic Extract

College of Horticulture, Northwest A&F University, Yangling 712100, China
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Author to whom correspondence should be addressed.
The authors contribute equally to this article.
Forests 2020, 11(1), 34; https://doi.org/10.3390/f11010034
Received: 14 October 2019 / Revised: 9 December 2019 / Accepted: 20 December 2019 / Published: 24 December 2019
(This article belongs to the Special Issue Biological and Bio-Based Management of Forest Pests and Pathogens)
Rot disease is a serious disease in pomegranate (Punica granatum L.) plantations in China. This disease usually weakens tree vigor, and seriously reduces the ornamental value, fruit yield, and quality. A better understanding of the pathogen that causes a disease is important for its control. Thus, the aims of this study were to isolate and identify the pathogen causing rot disease and to explore substances for its biological control. In this study, the morphology of the hyphae and spores of the pathogens was observed, and the pathogens were identified by morphological characteristics and the internal transcribed spacer (ITS) regions of their rDNA. Furthermore, the activity of an aqueous garlic extract as antifungal treatment for the identified pathogens was assessed. The results showed that the pathogens causing soft rot and dry rot in ‘Xinjiang Big Seed’ pomegranate were most probably Aspergillus niger and Botryosphaeria dothidea, respectively. In addition, the pathogenicity of A. niger was stronger than that of B. dothidea. The aqueous garlic extract had a strong antifungal effect on both pathogens by inhibiting mycelium growth in vitro, and the minimum inhibitory concentrations against A. niger and B. dothidea were 7.5 mg/mL and 10 mg/mL, respectively. View Full-Text
Keywords: pomegranate; rot disease; pathogen identification; antifungal activity; garlic extract pomegranate; rot disease; pathogen identification; antifungal activity; garlic extract
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Li, X.; Lu, X.; He, Y.; Deng, M.; Lv, Y. Identification the Pathogens Causing Rot Disease in Pomegranate (Punica granatum L.) in China and the Antifungal Activity of Aqueous Garlic Extract. Forests 2020, 11, 34.

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