Screening of Osteogenic-Enhancing Short Peptides from BMPs for Biomimetic Material Applications
Abstract
:1. Introduction
2. Results
2.1. Conditions of Peptide Array Screening
2.2. In Silico Screening to Determine Candidate Peptides
2.3. Peptide Array Screening for Osteogenic Proliferation Peptides
2.4. Peptide Array Screening for Osteogenic Differentiation Peptides
2.5. Determination of Osteogenic-Enhancing Peptides
3. Discussion
4. Materials and Methods
4.1. In Silico Screening for Candidate Peptides
4.2. Cell Culture
4.3. Peptide Array Synthesis
4.4. Cell Proliferation Assay Using the Peptide Array
4.5. ALP Activity Assay Using the Peptide Array
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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No. | Entry | Entry Name | Protein Names | Organism | Length |
---|---|---|---|---|---|
1 | Q90751 | BMP2_CHICK | Bone morphogenetic protein 2 (BMP-2) (fragment) | Gallus gallus (chicken) | 353 |
2 | O19006 | BMP2_DAMDA | Bone morphogenetic protein 2 (BMP-2) | Dama dama (fallow deer) (Cervus dama) | 396 |
3 | P12643 | BMP2_HUMAN | Bone morphogenetic protein 2 (BMP-2) (bone morphogenetic protein 2A) (BMP-2A) | Homo sapiens (human) | 396 |
4 | P21274 | BMP2_MOUSE | Bone morphogenetic protein 2 (BMP-2) (bone morphogenetic protein 2A) (BMP-2A) | Mus musculus (mouse) | 394 |
5 | O46564 | BMP2_RABIT | Bone morphogenetic protein 2 (BMP-2) | Oryctolagus cuniculus (rabbit) | 395 |
6 | P49001 | BMP2_RAT | Bone morphogenetic protein 2 (BMP-2) (bone morphogenetic protein 2A) (BMP-2A) | Rattus norvegicus (rat) | 393 |
7 | Q2KJH1 | BMP4_BOVIN | Bone morphogenetic protein 4 (BMP-4) | Bos taurus (bovine) | 409 |
8 | Q90752 | BMP4_CHICK | Bone morphogenetic protein 4 (BMP-4) | Gallus gallus (chicken) | 405 |
9 | Q29607 | BMP4_DAMDA | Bone morphogenetic protein 4 (BMP-4) | Dama dama (fallow deer) (Cervus dama) | 408 |
10 | P12644 | BMP4_HUMAN | Bone morphogenetic protein 4 (BMP-4) (bone morphogenetic protein 2B) (BMP-2B) | Homo sapiens (human) | 408 |
11 | P21275 | BMP4_MOUSE | Bone morphogenetic protein 4 (BMP-4) (bone morphogenetic protein 2B) (BMP-2B) | Mus musculus (mouse) | 408 |
12 | O46576 | BMP4_RABIT | Bone morphogenetic protein 4 (BMP-4) | Oryctolagus cuniculus (rabbit) | 409 |
13 | Q06826 | BMP4_RAT | Bone morphogenetic protein 4 (BMP-4) (bone morphogenetic protein 2B) (BMP-2B) | Rattus norvegicus (rat) | 408 |
14 | Q8MJV5 | BMP4_SUNMU | Bone morphogenetic protein 4 (BMP-4) (sBmp4) | Suncus murinus (Asian house shrew) (musk shrew) | 409 |
15 | P22004 | BMP6_HUMAN | Bone morphogenetic protein 6 (BMP-6) (VG-1-related protein) (VG-1-R) (VGR-1) | Homo sapiens (human) | 513 |
16 | P20722 | BMP6_MOUSE | Bone morphogenetic protein 6 (BMP-6) (VG-1-related protein) (VGR-1) | Mus musculus (mouse) | 510 |
17 | Q04906 | BMP6_RAT | Bone morphogenetic protein 6 (BMP-6) (VG-1-related protein) (VGR-1) | Rattus norvegicus (rat) | 506 |
18 | P18075 | BMP7_HUMAN | Bone morphogenetic protein 7 (BMP-7) (osteogenic protein 1) (OP-1) (eptotermin alfa) | Homo sapiens (human) | 431 |
19 | P23359 | BMP7_MOUSE | Bone morphogenetic protein 7 (BMP-7) (osteogenic protein 1) (OP-1) | Mus musculus (mouse) | 430 |
No. | Peptide Sequences |
---|---|
(1) | HRINIYEII |
(2) | TRLLDTRLV |
(3) | DVTPAVMRW |
(4) | NHGFVVEVT |
(5) | VEVTHLEEK |
(6) | RHVRISRSL |
(7) | SWSQIRPLL |
(8) | RPLLVTFGH |
(9) | TFGHDGKGH |
(10) | LYVDFSDVG |
(11) | SDVGWNDWI |
(12) | NDWIVAPPG |
(13) | AFYCHGECP |
(14) | GECPFPLAD |
(15) | PLADHLNST |
(16) | LNSTNHAIV |
(17) | HAIVQTLVN |
(18) | TLVNSVNSK |
(19) | VNSKIPKAC |
(20) | PKACCVPTE |
(21) | VPTELSAIS |
(22) | SAISMLYLD |
(23) | EKVVLKNYQ |
(24) | KNYQDMVVE |
(25) | MVEEGCGCR |
RGD | |
Blank (no peptide) |
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Kanie, K.; Kurimoto, R.; Tian, J.; Ebisawa, K.; Narita, Y.; Honda, H.; Kato, R. Screening of Osteogenic-Enhancing Short Peptides from BMPs for Biomimetic Material Applications. Materials 2016, 9, 730. https://doi.org/10.3390/ma9090730
Kanie K, Kurimoto R, Tian J, Ebisawa K, Narita Y, Honda H, Kato R. Screening of Osteogenic-Enhancing Short Peptides from BMPs for Biomimetic Material Applications. Materials. 2016; 9(9):730. https://doi.org/10.3390/ma9090730
Chicago/Turabian StyleKanie, Kei, Rio Kurimoto, Jing Tian, Katsumi Ebisawa, Yuji Narita, Hiroyuki Honda, and Ryuji Kato. 2016. "Screening of Osteogenic-Enhancing Short Peptides from BMPs for Biomimetic Material Applications" Materials 9, no. 9: 730. https://doi.org/10.3390/ma9090730