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Optimization of Supercritical Fluid Extraction of Total Alkaloids, Peimisine, Peimine and Peiminine from the Bulb of Fritillaria thunbergii Miq, and Evaluation of Antioxidant Activities of the Extracts

Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China
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Academic Editor: Carlos Manuel Silva
Materials 2016, 9(7), 524; https://doi.org/10.3390/ma9070524
Received: 31 March 2016 / Revised: 23 June 2016 / Accepted: 24 June 2016 / Published: 29 June 2016
(This article belongs to the Special Issue Optimisation and Scale-Up of Supercritical Fluid Extraction Processes)
Supercritical fluid extraction (SFE) was used to extract total alkaloids, peimisine, peimine and peiminine from the bulb of Fritillaria thunbergii Miq. The antioxidant capacity of the extracts was evaluated by DPPH radical scavenging activity (DPPH-RSA), ABTS radical scavenging activity (ABTS-RSA) and ferric reducing capacity (FRAP) assay. A central composite design (CCD) with four variables and five levels was employed for optimization of process parameters, and response surface plots were constructed in accordance with a second order polynomial model. Under optimal conditions of 3.0 h, 60.4 °C, 26.5 MPa and 89.3% ethanol, the highest yields were predicted to be 3.8 mg/g for total alkaloids, 0.5 mg/g for peimisine, 1.3 mg/g for peimine and 1.3 mg/g for peiminine, and the antioxidant capacity of extracts displayed EC50, DPPH value of 5.5 mg/mL, EC50, ABTS value of 0.3 mg/mL and FRAP value of 118.2 mg ascorbic acid equivalent (AAE)/100 g. View Full-Text
Keywords: Fritillaria thunbergii Miq; supercritical fluid extraction; central composite design; total alkaloids; peimisine; peimine; peiminine; antioxidant capacity Fritillaria thunbergii Miq; supercritical fluid extraction; central composite design; total alkaloids; peimisine; peimine; peiminine; antioxidant capacity
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Ruan, X.; Yang, L.; Cui, W.-X.; Zhang, M.-X.; Li, Z.-H.; Liu, B.; Wang, Q. Optimization of Supercritical Fluid Extraction of Total Alkaloids, Peimisine, Peimine and Peiminine from the Bulb of Fritillaria thunbergii Miq, and Evaluation of Antioxidant Activities of the Extracts. Materials 2016, 9, 524.

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