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Open AccessArticle

Strontium-Substituted Bioceramics Particles: A New Way to Modulate MCP-1 and Gro-α Production by Human Primary Osteoblastic Cells

EA 4691 Biomatériaux et Inflammation en Site Osseux (BIOS), SFR CAP-Santé (FED 4231), University of Reims-Champagne-Ardenne, 1 Avenue du Maréchal Juin, 51095 Reims CEDEX, France
UFR Odontologie, University of Reims Champagne Ardenne, 2 rue du Général Koenig, 51100 Reims, France
University Hospital of Reims, 45 rue Cognacq Jay, 51100 Reims, France
UFR de Pharmacie, University of Reims Champagne Ardenne, 1 Avenue du Maréchal Juin, 51095 Reims CEDEX, France
Laboratoire de Physique Corpusculaire de Clermont-Ferrand, UMR 6533, CNRS/IN2P3, Université Clermont Auvergne, 24 Avenue des Landais, 63177 Aubiere CEDEX, France
Institut de Chimie de Clermont-Ferrand (ICCF), UMR 6296, CNRS, Université Clermont Auvergne, SIGMA Clermont, F-63000 Clermont-Ferrand, France
Institut Pluridisciplinaire Hubert Curien (IPHC), UMR 7178, CNRS, Université de Strasbourg, 23 rue de Loess, F-67000 Strasbourg, France
Author to whom correspondence should be addressed.
Those authors have contributed equally to this manuscript.
Academic Editors: Andrew J. Ruys and Aldo R. Boccaccini
Materials 2016, 9(12), 985;
Received: 29 September 2016 / Revised: 8 November 2016 / Accepted: 25 November 2016 / Published: 5 December 2016
(This article belongs to the Special Issue Calcium Phosphate in Biomedical Applications)
Background: To avoid morbidity and limited availability associated with autografts, synthetic calcium phosphate (CaP) ceramics were extensively developed and used as bone filling materials. Controlling their induced-inflammatory response nevertheless remained a major concern. Strontium-containing CaP ceramics were recently demonstrated for impacting cytokines’ secretion pattern of human primary monocytes. The present study focuses on the ability of strontium-containing CaP to control the human primary bone cell production of two major inflammatory and pro-osteoclastogenic mediators, namely MCP-1 and Gro-α, in response to ceramics particles. Methods: This in vitro study was performed using human primary osteoblasts in which their response to ceramics was evaluated by PCR arrays, antibody arrays were used for screening and real-time PCR and ELISA for more focused analyses. Results: Study of mRNA and protein expression highlights that human primary bone cells are able to produce these inflammatory mediators and reveal that the adjunction of CaP in the culture medium leads to their enhanced production. Importantly, the current work determines the down-regulating effect of strontium-substituted CaP on MCP-1 and Gro-α production. Conclusion: Our findings point out a new capability of strontium to modulate human primary bone cells’ communication with the immune system. View Full-Text
Keywords: human primary bone cells; cytokines; strontium; calcium-phosphate human primary bone cells; cytokines; strontium; calcium-phosphate
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Braux, J.; Velard, F.; Guillaume, C.; Jourdain, M.-L.; Gangloff, S.C.; Jallot, E.; Nedelec, J.-M.; Laquerrière, P.; Laurent-Maquin, D. Strontium-Substituted Bioceramics Particles: A New Way to Modulate MCP-1 and Gro-α Production by Human Primary Osteoblastic Cells. Materials 2016, 9, 985.

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