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Materials 2015, 8(10), 7217-7229;

Active Nanofibrous Membrane Effects on Gingival Cell Inflammatory Response

INSERM (French National Institute of Health and Medical Research), UMR 1109, Osteoarticular and Dental Regenerative Nanomedicine laboratory, Faculté de Médecine, FMTS, Strasbourg Cedex F-67085, France
Department of Periodontology, University of Strasbourg, Dental Faculty, 8 rue Sainte-Elisabeth, Strasbourg F-67000, France
Author to whom correspondence should be addressed.
Academic Editor: Marco Salerno
Received: 25 August 2015 / Revised: 8 October 2015 / Accepted: 20 October 2015 / Published: 27 October 2015
(This article belongs to the Special Issue Therapeutics Delivery Systems for Regenerative Nanomedicine)
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Alpha-melanocyte stimulating hormone (α-MSH) is involved in normal skin wound healing and also has anti-inflammatory properties. The association of α-MSH to polyelectrolyte layers with various supports has been shown to improve these anti-inflammatory properties. This study aimed to evaluate the effects of nanofibrous membrane functionalized with α-MSH linked to polyelectrolyte layers on gingival cell inflammatory response. Human oral epithelial cells (EC) and fibroblasts (FB) were cultured on plastic or electrospun Poly-#-caprolactone (PCL) membranes with α-MSH covalently coupled to Poly-L-glutamic acid (PGA-α-MSH), for 6 to 24 h. Cells were incubated with or without Porphyromonas gingivalis lipopolysaccharide (Pg-LPS). Cell proliferation and migration were determined using AlamarBlue test and scratch assay. Expression of interleukin-6 (IL-6), tumor necrosis factor (TNF-α), and transforming growth factor-beta (TGF-β) was evaluated using RT-qPCR method. Cell cultures on plastic showed that PGA-α-MSH reduced EC and FB migration and decreased IL-6 and TGF-β expression in Pg-LPS stimulated EC. PGA-α-MSH functionalized PCL membranes reduced proliferation of Pg-LPS stimulated EC and FB. A significant decrease of IL-6, TNF-α, and TGF-β expression was also observed in Pg-LPS stimulated EC and FB. This study showed that the functionalization of nanofibrous PCL membranes efficiently amplified the anti-inflammatory effect of PGA-α-MSH on gingival cells. View Full-Text
Keywords: anti-inflammatory agents; lipopolysaccharides; fibroblasts; epithelial cells; polycaprolactone; cytokines anti-inflammatory agents; lipopolysaccharides; fibroblasts; epithelial cells; polycaprolactone; cytokines

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Morand, D.-N.; Huck, O.; Keller, L.; Jessel, N.; Tenenbaum, H.; Davideau, J.-L. Active Nanofibrous Membrane Effects on Gingival Cell Inflammatory Response. Materials 2015, 8, 7217-7229.

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