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Article

Antibacterial Effect of Amino Acid–Silver Complex Loaded Montmorillonite Incorporated in Dental Acrylic Resin

1
Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Kagawa 761-0395, Japan
2
Department of Pathology & Experimental Medicine, Dentistry and Pharmaceutical Sciences, Graduate School of Medicine, Okayama University Hospital, Okayama University, Okayama 700-8558, Japan
3
Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School, Okayama 700-8558, Japan
4
Department of Biomaterials and Bioengineering, Faculty of Dental Medicine, Hokkaido University, Hokkaido 060-8586, Japan
5
KU Leuven (University of Leuven) Department of Oral Health Research, BIOMAT & University Hospitals Leuven, B-3000 Leuven, Belgium
*
Author to whom correspondence should be addressed.
Academic Editor: Javier Gil
Materials 2021, 14(6), 1442; https://doi.org/10.3390/ma14061442
Received: 6 February 2021 / Revised: 8 March 2021 / Accepted: 10 March 2021 / Published: 16 March 2021
(This article belongs to the Special Issue Materials for Hard Tissue Repair and Regeneration)
Several dental materials contain silver for antibacterial effect, however the effect is relatively low. The reason for the lower antibacterial efficacy of silver is considered to be the fact that silver ions bind to chloride ions in saliva. To develop new effective silver antibacterial agents that can be useful in the mouth, we synthesized two novel amino acid (methionine or histidine)–silver complexes (Met or His–Ag) loaded with montmorillonite (Mont) and analyzed their antibacterial efficacy. At first the complexes were characterized using nuclear magnetic resonance (NMR), and amino acid–Ag complex-loaded Mont (amino acid–Ag–Mont) were characterized using X-ray diffraction (XRD) and scanning electron microscopy (SEM). The antibacterial efficacy of these materials in dental acrylic resin was then investigated by bacterial growth measurement using a spectrophotometer. As controls, commercially available silver-loaded zeolite and silver-zirconium phosphate were also tested. Dental acrylic resin incorporating His–Ag–Mont strongly inhibited Streptococcus mutans growth. This was explained by the fact that His-Ag complex revealed the highest amounts of silver ions in the presence of chloride. The structure of the amino acid–Ag complexes affected the silver ion presence in chloride and the antibacterial efficacy. His–Ag–Mont might be used as antibacterial agents for dental materials. View Full-Text
Keywords: montmorillonite; amino acid; antibacterial; Streptococcus mutans; nuclear magnetic resonance; X-ray diffraction montmorillonite; amino acid; antibacterial; Streptococcus mutans; nuclear magnetic resonance; X-ray diffraction
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MDPI and ACS Style

Yoshihara, K.; Nagaoka, N.; Umeno, A.; Sonoda, A.; Obika, H.; Yoshida, Y.; Van Meerbeek, B.; Makita, Y. Antibacterial Effect of Amino Acid–Silver Complex Loaded Montmorillonite Incorporated in Dental Acrylic Resin. Materials 2021, 14, 1442. https://doi.org/10.3390/ma14061442

AMA Style

Yoshihara K, Nagaoka N, Umeno A, Sonoda A, Obika H, Yoshida Y, Van Meerbeek B, Makita Y. Antibacterial Effect of Amino Acid–Silver Complex Loaded Montmorillonite Incorporated in Dental Acrylic Resin. Materials. 2021; 14(6):1442. https://doi.org/10.3390/ma14061442

Chicago/Turabian Style

Yoshihara, Kumiko, Noriyuki Nagaoka, Aya Umeno, Akinari Sonoda, Hideki Obika, Yasuhiro Yoshida, Bart Van Meerbeek, and Yoji Makita. 2021. "Antibacterial Effect of Amino Acid–Silver Complex Loaded Montmorillonite Incorporated in Dental Acrylic Resin" Materials 14, no. 6: 1442. https://doi.org/10.3390/ma14061442

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