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Materials
Volume 14
Issue 5
10.3390/ma14051119
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Article
Peer-Review Record

Use of Therapeutic Pathogen Recognition Receptor Ligands for Osteo-Immunomodulation

Materials 2021, 14(5), 1119; https://doi.org/10.3390/ma14051119
by Paree Khokhani 1,†, Nada R. Rahmani 1,†, Anne Kok 1, F. Cumhur Öner 1, Jacqueline Alblas 1, Harrie Weinans 1,2, Moyo C. Kruyt 1 and Michiel Croes 1,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Reviewer 3: Anonymous
Materials 2021, 14(5), 1119; https://doi.org/10.3390/ma14051119
Submission received: 2 February 2021 / Accepted: 22 February 2021 / Published: 27 February 2021
(This article belongs to the Special Issue Multifunctional Coatings for Bone Regenerative Medicine)

Round 1

Reviewer 1 Report

The manuscript has quite improved from the previous submission.
Therefore I think it can be accepted in the present form.

Congratulations for the work improvement.

Reviewer 2 Report

All concerns have been addressed.

The manuscript is ready to publish.

Reviewer 3 Report

The authors of the paper “Use of Therapeutic Pathogen Recognition Receptor Ligands for Osteo-immunomodulation” aimed to explore the response of human mesenchymal stem cells (hMSCs) and monocytes-key cells in bone regeneration- towards therapeutic pathogen recognition receptor (PRR) ligand stimulation. Therefore they have evaluated the effect of various synthetic PRR ligands on bone cell differentiation and inflammation: alkaline phosphatase (ALP) activity in hMSCs, osteoclastogenic response induced in monocytes measured by TRAP, inflammatory property of ligands by ELISA evaluation of pro and anti–inflammatory (TNF-alpha, IL-6, IL-8, IL-10) cytokines expression. Based on the complex investigation, nucleic acid-based ligands Poly (I:C) and CpG ODN C were found to increase early ALP activity and not interfering with osteoclasts formation process. Their pro-osteogenic action was associated with a mild pro-inflammatory cell response. Due to these osteo-immunomodulatory effects, nucleic-acid ligands can be promising candidates for multifunctional coatings.

The new form is better presented.

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