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Open AccessArticle

Effect of UV and Gamma Irradiation Sterilization Processes in the Properties of Different Polymeric Nanoparticles for Biomedical Applications

1
Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón, Ciudad de México 11340, Mexico
2
Laboratorio de Medicina Genómica, Departamento de Genética (CENIAQ), Instituto Nacional de Rehabilitación-Luis Guillermo Ibarra Ibarra (INR-LGII), Ciudad de México 14389, Mexico
3
Laboratorio de Tecnología Farmacéutica, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Cuautitlán Izcalli, Edo. de México 54740, Mexico
4
Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México 04510, Mexico
5
Programa de Ciencias Biomédicas, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México 04510, Mexico
6
Departamento de Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Edificio “A” 3er piso, Circuito Interior, Avenida Universidad 3000, Ciudad Universitaria, Coyoacán, Ciudad de México 04510, Mexico
7
Unidad de Microscopía, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Edificio “A” planta baja, Circuito Interior, Avenida Universidad 3000, Ciudad Universitaria, Coyoacán, Ciudad de México 04510, Mexico
8
Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados (CINVESTAV-IPN), Ciudad de México 07360, Mexico
9
Departamento de Infectología, (CENIAQ), Instituto Nacional de Rehabilitación-Luis Guillermo Ibarra Ibarra, Ciudad de México (CDMX) 14389, Mexico
10
Escuela de Ingeniería, Departamento de Biotecnología, Instituto Tecnológico y de Estudios Superiores de Monterrey-Campus, Ciudad de México 14380, Mexico
*
Authors to whom correspondence should be addressed.
These authors equally contributed to the work.
Materials 2020, 13(5), 1090; https://doi.org/10.3390/ma13051090
Received: 31 December 2019 / Revised: 19 February 2020 / Accepted: 25 February 2020 / Published: 1 March 2020
(This article belongs to the Special Issue Micro and Nanotechnologies in Biomedicines)
The sterilization processes of nanoparticles (NP) by autoclaving and filtration are two of the most utilized methods in the pharmaceutical industry but are not always a viable option. For this reason, the search for alternative options such as UV and gamma radiation is of interest. In this work, we evaluated both types of sterilization on two types of NP in solid state widely employed in the literature for biomedical applications, poly-(ε-caprolactone) and poly(d,l-lactide-co-glycolide) acid NP stabilized with polyvinyl alcohol. Physicochemical properties and cell viability were studied pre- and post-sterilization. The efficiency of irradiation sterilization was performed by a test of sterility using 1 × 108 CFU/mL of Escherichia coli, Staphylococcus aureus, and Candida albicans. Microbiological monitoring revealed that both methods were sufficient for sterilization. After the UV irradiation sterilization (100 µJ/cm2), no substantial changes were observed in the physicochemical properties of the NP or in the interaction or morphology of human glial cells, though 5 and 10 kGy of gamma irradiation showed slight changes of NP size as well as a decrease in cell viability (from 100 µg/mL of NP). At 5 kGy of radiation doses, the presence of trehalose as cryoprotectant reduces the cell damage with high concentrations of NP, but this did not occur at 10 kGy. Therefore, these methods could be highly effective and low-processing-time options for sterilizing NP for medical purposes. However, we suggest validating each NP system because these generally are of different polymer-composition systems. View Full-Text
Keywords: nanoparticles; sterilization; ultraviolet radiation; gamma radiation; poly (d,l-lactide-co-glycolide) acid; poly-(ε-caprolactone) nanoparticles; sterilization; ultraviolet radiation; gamma radiation; poly (d,l-lactide-co-glycolide) acid; poly-(ε-caprolactone)
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MDPI and ACS Style

Tapia-Guerrero, Y.S.; Del Prado-Audelo, M.L.; Borbolla-Jiménez, F.V.; Giraldo Gomez, D.M.; García-Aguirre, I.; Colín-Castro, C.A.; Morales-González, J.A.; Leyva-Gómez, G.; Magaña, J.J. Effect of UV and Gamma Irradiation Sterilization Processes in the Properties of Different Polymeric Nanoparticles for Biomedical Applications. Materials 2020, 13, 1090. https://doi.org/10.3390/ma13051090

AMA Style

Tapia-Guerrero YS, Del Prado-Audelo ML, Borbolla-Jiménez FV, Giraldo Gomez DM, García-Aguirre I, Colín-Castro CA, Morales-González JA, Leyva-Gómez G, Magaña JJ. Effect of UV and Gamma Irradiation Sterilization Processes in the Properties of Different Polymeric Nanoparticles for Biomedical Applications. Materials. 2020; 13(5):1090. https://doi.org/10.3390/ma13051090

Chicago/Turabian Style

Tapia-Guerrero, Y. S.; Del Prado-Audelo, M. L.; Borbolla-Jiménez, F. V.; Giraldo Gomez, D. M.; García-Aguirre, I.; Colín-Castro, C. A.; Morales-González, J. A.; Leyva-Gómez, G.; Magaña, J. J. 2020. "Effect of UV and Gamma Irradiation Sterilization Processes in the Properties of Different Polymeric Nanoparticles for Biomedical Applications" Materials 13, no. 5: 1090. https://doi.org/10.3390/ma13051090

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