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Article

Effect of Multi-Phosphonate Coating of Titanium Surfaces on Osteogenic Potential

1
Division of Conservative Dentistry and Periodontology, University Clinic of Dentistry, Medical University of Vienna, 1090 Vienna, Austria
2
Competence Center for Periodontal Research, University Clinic of Dentistry, Medical University of Vienna, 1090 Vienna, Austria
3
Division of Orthodontics, University Clinic of Dentistry, Medical University of Vienna, 1090 Vienna, Austria
*
Author to whom correspondence should be addressed.
Materials 2020, 13(24), 5777; https://doi.org/10.3390/ma13245777
Received: 6 November 2020 / Revised: 14 December 2020 / Accepted: 15 December 2020 / Published: 17 December 2020
(This article belongs to the Special Issue Materials in Implant Dentistry and Regenerative Medicine)
The aim of this study was to evaluate the impact of a novel multi-phosphonate (MP) coating strategy of dental implant surfaces on the expression of osteogenesis-related factors in vitro. MG-63 human osteoblast-like cells, bone marrow mesenchymal stem cells (BM-MSCs), and human periodontal ligament stem cells (hPDLSCs) were cultured separately on titanium disks with and without MP coating. Cell attachment was visualized by focal adhesion and actin cytoskeleton staining. The proliferation and gene expression of the markers related to osteogenesis and bone turnover were measured after 48 and 120 h of cell culture. Actin cytoskeleton assembly and focal adhesion were similar between test surfaces within each cell type but differed from those on tissue culture plastic (TCP). The proliferation of MG-63 cells and PDLSCs was comparable on all surfaces, while BM-MSCs showed an increase on tissue culture plastic (TCP) versus titanium. The gene expression of osteoprotegerin and receptor activator of nuclear factor-kappa B ligand was higher in MG-63 cells grown on MP-coated surfaces. At the same time, osteocalcin was decreased compared to the other surfaces. Collagen type I gene expression after 120 h was significantly lower in hPDLSCs cultivated on MP-coated surfaces. Within the limitations of this study, MP coating on titanium surfaces might have a slight beneficial effect on bone turnover in vitro. View Full-Text
Keywords: implant titanium surface; surface modification; multi-phosphonate coating; osseointegration; osteoblast-like cells; mesenchymal stem cells; periodontal ligament stem cells implant titanium surface; surface modification; multi-phosphonate coating; osseointegration; osteoblast-like cells; mesenchymal stem cells; periodontal ligament stem cells
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MDPI and ACS Style

Wehner, C.; Behm, C.; Husejnagic, S.; Moritz, A.; Rausch-Fan, X.; Andrukhov, O. Effect of Multi-Phosphonate Coating of Titanium Surfaces on Osteogenic Potential. Materials 2020, 13, 5777. https://doi.org/10.3390/ma13245777

AMA Style

Wehner C, Behm C, Husejnagic S, Moritz A, Rausch-Fan X, Andrukhov O. Effect of Multi-Phosphonate Coating of Titanium Surfaces on Osteogenic Potential. Materials. 2020; 13(24):5777. https://doi.org/10.3390/ma13245777

Chicago/Turabian Style

Wehner, Christian, Christian Behm, Selma Husejnagic, Andreas Moritz, Xiaohui Rausch-Fan, and Oleh Andrukhov. 2020. "Effect of Multi-Phosphonate Coating of Titanium Surfaces on Osteogenic Potential" Materials 13, no. 24: 5777. https://doi.org/10.3390/ma13245777

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