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Article

Dynamic Characterization of the Biomechanical Behaviour of Bovine Ovarian Cortical Tissue and Its Short-Term Effect on Ovarian Tissue and Follicles

by
Giulia Pascoletti
1,
Maddalena Di Nardo
2,
Gionata Fragomeni
3,
Vincenza Barbato
2,
Teresa Capriglione
2,†,
Roberto Gualtieri
2,
Riccardo Talevi
2,
Gerardo Catapano
4 and
Elisabetta M. Zanetti
1,*
1
Department of Engineering, University of Perugia, 06125 Perugia, Italy
2
Department of Biology, University of Naples Federico II, 80100 Naples, Italy
3
Department of Medical and Surgical Sciences, Magna Graecia University, 88100 Catanzaro, Italy
4
Department of Mechanical, Energy and Management Engineering, University of Calabria, 87030 Rende (CS), Italy
*
Author to whom correspondence should be addressed.
†
In loving memory.
Materials 2020, 13(17), 3759; https://doi.org/10.3390/ma13173759
Submission received: 9 July 2020 / Revised: 17 August 2020 / Accepted: 20 August 2020 / Published: 25 August 2020
(This article belongs to the Special Issue Novel Load Systems for In Vitro Testing of Biomaterials and Medical Devices)
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Abstract

The ovary is a dynamic mechanoresponsive organ. In vitro, tissue biomechanics was reported to affect follicle activation mainly through the Hippo pathway. Only recently, ovary responsiveness to mechanical signals was exploited for reproductive purposes. Unfortunately, poor characterization of ovarian cortex biomechanics and of the mechanical challenge hampers reproducible and effective treatments, and prevention of tissue damages. In this study the biomechanical response of ovarian cortical tissue from abattoir bovines was characterized for the first time. Ovarian cortical tissue fragments were subjected to uniaxial dynamic testing at frequencies up to 30 Hz, and at increasing average stresses. Tissue structure prior to and after testing was characterized by histology, with established fixation and staining protocols, to assess follicle quality and stage. Tissue properties largely varied with the donor. Bovine ovarian cortical tissue consistently exhibited a nonlinear viscoelastic behavior, with dominant elastic characteristics, in the low range of other reproductive tissues, and significant creep. Strain rate was independent of the applied stress. Histological analysis prior to and after mechanical tests showed that the short-term dynamic mechanical test used for the study did not cause significant tissue tear, nor follicle expulsion or cell damage.
Keywords: ovarian tissue; creep; elastic modulus; tensile test; viscous behavior; biomechanics ovarian tissue; creep; elastic modulus; tensile test; viscous behavior; biomechanics
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MDPI and ACS Style

Pascoletti, G.; Di Nardo, M.; Fragomeni, G.; Barbato, V.; Capriglione, T.; Gualtieri, R.; Talevi, R.; Catapano, G.; Zanetti, E.M. Dynamic Characterization of the Biomechanical Behaviour of Bovine Ovarian Cortical Tissue and Its Short-Term Effect on Ovarian Tissue and Follicles. Materials 2020, 13, 3759. https://doi.org/10.3390/ma13173759

AMA Style

Pascoletti G, Di Nardo M, Fragomeni G, Barbato V, Capriglione T, Gualtieri R, Talevi R, Catapano G, Zanetti EM. Dynamic Characterization of the Biomechanical Behaviour of Bovine Ovarian Cortical Tissue and Its Short-Term Effect on Ovarian Tissue and Follicles. Materials. 2020; 13(17):3759. https://doi.org/10.3390/ma13173759

Chicago/Turabian Style

Pascoletti, Giulia, Maddalena Di Nardo, Gionata Fragomeni, Vincenza Barbato, Teresa Capriglione, Roberto Gualtieri, Riccardo Talevi, Gerardo Catapano, and Elisabetta M. Zanetti. 2020. "Dynamic Characterization of the Biomechanical Behaviour of Bovine Ovarian Cortical Tissue and Its Short-Term Effect on Ovarian Tissue and Follicles" Materials 13, no. 17: 3759. https://doi.org/10.3390/ma13173759

APA Style

Pascoletti, G., Di Nardo, M., Fragomeni, G., Barbato, V., Capriglione, T., Gualtieri, R., Talevi, R., Catapano, G., & Zanetti, E. M. (2020). Dynamic Characterization of the Biomechanical Behaviour of Bovine Ovarian Cortical Tissue and Its Short-Term Effect on Ovarian Tissue and Follicles. Materials, 13(17), 3759. https://doi.org/10.3390/ma13173759

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