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Article

Cytotoxicity and Gene Expression Changes of a Novel Homeopathic Antiseptic Oral Rinse in Comparison to Chlorhexidine in Gingival Fibroblasts

1
Department of Cranio-Maxillofacial Surgery, Inselspital, Bern University Hospital, University of Bern, 3010 Bern, Switzerland
2
Private Practice, Pikos Institute, Trinity, FL 34655, USA
3
Department of Periodontology, University of Bern, 3010 Bern, Switzerland
*
Author to whom correspondence should be addressed.
Materials 2020, 13(14), 3190; https://doi.org/10.3390/ma13143190
Received: 11 June 2020 / Revised: 10 July 2020 / Accepted: 14 July 2020 / Published: 17 July 2020
Most available antiseptic solutions available today have strong antibacterial effects, however most also possess major cytotoxic effects on human gingival tissues. The VEGA Oral Care Recovery Kit (StellaLife), previously evaluated in clinical studies, consists of 16 active ingredients that are monographed in the Homeopathic Pharmacopeia of United States (HPUS) and recognized for their accelerated healing properties (reduction in post-op pain). The aim of this study was to compare VEGA to chlorhexidine (CHX) in vitro on gingival fibroblast viability, survival at various concentrations, migration assay, proliferation activity, expression of both regenerative growth factors as well as inflammatory markers, and collagen synthesis. A 10-fold dilution of standard CHX (0.02%) led to cell death, whereas cell viability was significantly better in the VEGA group for all tested parameters. Furthermore, VEGA also induced significantly greater fibroblast migration and proliferation. CHX negatively impacted the cellular inflammatory response of gingival fibroblasts, and also led to a reduction in collagen synthesis (50% decrease). Findings from the present study provide support from basic laboratory experiments that validate the previous clinical studies supporting the use of the VEGA oral rinse on its superior biocompatibility and wound healing properties when compared to CHX. View Full-Text
Keywords: chlorhexidine; anti-septic solution; cell viability; periodontal disease; oral rinses chlorhexidine; anti-septic solution; cell viability; periodontal disease; oral rinses
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MDPI and ACS Style

Fujioka-Kobayashi, M.; Schaller, B.; Pikos, M.A.; Sculean, A.; Miron, R.J. Cytotoxicity and Gene Expression Changes of a Novel Homeopathic Antiseptic Oral Rinse in Comparison to Chlorhexidine in Gingival Fibroblasts. Materials 2020, 13, 3190. https://doi.org/10.3390/ma13143190

AMA Style

Fujioka-Kobayashi M, Schaller B, Pikos MA, Sculean A, Miron RJ. Cytotoxicity and Gene Expression Changes of a Novel Homeopathic Antiseptic Oral Rinse in Comparison to Chlorhexidine in Gingival Fibroblasts. Materials. 2020; 13(14):3190. https://doi.org/10.3390/ma13143190

Chicago/Turabian Style

Fujioka-Kobayashi, Masako, Benoit Schaller, Michael A. Pikos, Anton Sculean, and Richard J. Miron 2020. "Cytotoxicity and Gene Expression Changes of a Novel Homeopathic Antiseptic Oral Rinse in Comparison to Chlorhexidine in Gingival Fibroblasts" Materials 13, no. 14: 3190. https://doi.org/10.3390/ma13143190

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