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Article

In Vitro Mechanical and Biological Properties of 3D Printed Polymer Composite and β-Tricalcium Phosphate Scaffold on Human Dental Pulp Stem Cells

1
Department of Oral and Cranio-Maxillofacial Surgery, University Hospital Basel, Spitalstrasse 21, 4031 Basel, Switzerland
2
Medical Additive Manufacturing Research Group, Department of Biomedical Engineering, University of Basel, Gewerbestrasse 16, 4123 Allschwil, Switzerland
3
Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), 50, UNIST-gil, Ulsan 44919, Korea
*
Authors to whom correspondence should be addressed.
Materials 2020, 13(14), 3057; https://doi.org/10.3390/ma13143057
Received: 18 May 2020 / Revised: 26 June 2020 / Accepted: 6 July 2020 / Published: 8 July 2020
(This article belongs to the Special Issue 3D-Printed Dental Materials)
3D printed biomaterials have been extensively investigated and developed in the field of bone regeneration related to clinical issues. However, specific applications of 3D printed biomaterials in different dental areas have seldom been reported. In this study, we aimed to and successfully fabricated 3D poly (lactic-co-glycolic acid)/β-tricalcium phosphate (3D-PLGA/TCP) and 3D β-tricalcium phosphate (3D-TCP) scaffolds using two relatively distinct 3D printing (3DP) technologies. Conjunctively, we compared and investigated mechanical and biological responses on human dental pulp stem cells (hDPSCs). Physicochemical properties of the scaffolds, including pore structure, chemical elements, and compression modulus, were characterized. hDPSCs were cultured on scaffolds for subsequent investigations of biocompatibility and osteoconductivity. Our findings indicate that 3D printed PLGA/TCP and β-tricalcium phosphate (β-TCP) scaffolds possessed a highly interconnected and porous structure. 3D-TCP scaffolds exhibited better compressive strength than 3D-PLGA/TCP scaffolds, while the 3D-PLGA/TCP scaffolds revealed a flexible mechanical performance. The introduction of 3D structure and β-TCP components increased the adhesion and proliferation of hDPSCs and promoted osteogenic differentiation. In conclusion, 3D-PLGA/TCP and 3D-TCP scaffolds, with the incorporation of hDPSCs as a personalized restoration approach, has a prospective potential to repair minor and critical bone defects in oral and maxillofacial surgery, respectively. View Full-Text
Keywords: 3D printing; dental biomaterials; polymer printing; ceramic printing; human dental pulp stem cell; in vitro research; bone regeneration 3D printing; dental biomaterials; polymer printing; ceramic printing; human dental pulp stem cell; in vitro research; bone regeneration
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MDPI and ACS Style

Cao, S.; Han, J.; Sharma, N.; Msallem, B.; Jeong, W.; Son, J.; Kunz, C.; Kang, H.-W.; Thieringer, F.M. In Vitro Mechanical and Biological Properties of 3D Printed Polymer Composite and β-Tricalcium Phosphate Scaffold on Human Dental Pulp Stem Cells. Materials 2020, 13, 3057. https://doi.org/10.3390/ma13143057

AMA Style

Cao S, Han J, Sharma N, Msallem B, Jeong W, Son J, Kunz C, Kang H-W, Thieringer FM. In Vitro Mechanical and Biological Properties of 3D Printed Polymer Composite and β-Tricalcium Phosphate Scaffold on Human Dental Pulp Stem Cells. Materials. 2020; 13(14):3057. https://doi.org/10.3390/ma13143057

Chicago/Turabian Style

Cao, Shuaishuai, Jonghyeuk Han, Neha Sharma, Bilal Msallem, Wonwoo Jeong, Jeonghyun Son, Christoph Kunz, Hyun-Wook Kang, and Florian M. Thieringer 2020. "In Vitro Mechanical and Biological Properties of 3D Printed Polymer Composite and β-Tricalcium Phosphate Scaffold on Human Dental Pulp Stem Cells" Materials 13, no. 14: 3057. https://doi.org/10.3390/ma13143057

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