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Article

Adsorption and Release of Growth Factors from Four Different Porcine-Derived Collagen Matrices

by 1,2,†, 1,2,3,†, 2 and 1,2,*
1
Laboratory of Oral Cell Biology, Dental Research Center, School of Dental Medicine, University of Bern, Freiburgstrasse 3, 3010 Bern, Switzerland
2
Department of Periodontology, School of Dental Medicine, University of Bern, Freiburgstrasse 7, 3010 Bern, Switzerland
3
Department of Periodontology, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiaotong University, Zhizaoju Road 639, Shanghai 200011, China
*
Author to whom correspondence should be addressed.
Equal contribution.
Materials 2020, 13(11), 2635; https://doi.org/10.3390/ma13112635
Received: 8 May 2020 / Revised: 28 May 2020 / Accepted: 2 June 2020 / Published: 9 June 2020
Xenogeneic acellular collagen matrices represent a safe alternative to autologous soft tissue transplants in periodontology and implant dentistry. Here, we aimed to investigate the adsorption and release of growth factors from four porcine-derived collagen matrices using enzyme-linked immunosorbent assay. Non-crosslinked collagen matrix (NCM), crosslinked collagen matrix (CCM), dried acellular dermal matrix (DADM), and hydrated acellular dermal matrix (HADM) adsorbed each of the following growth factors, TGF-β1, FGF-2, PDGF-BB, GDF-5 and BMP-2, with an efficiency close to 100%. Growth factor release for a 13-day period was in the range of 10–50% of the adsorbed protein, except for the BMP-2 release that was in the range of 5–7%. Generally, protein release occurred in two phases. Phase I was arbitrary defined by the highest release from the matrices, usually within 24 h. Phase II, spanning the period immediately after the peak release until day 13, corresponded to the delayed release of the growth factors from the deeper layers of the matrices. HADM showed significantly (P < 0.001) higher TGF-β1, FGF-2, and PDGF-BB release in phase II, compared to the rest of the matrices. NCM exhibited significantly (P < 0.001) higher FGF-2 release in phase II, compared to CCM and DADM as well as a characteristic second peak in PDGF-BB release towards the middle of the tested period. In contrast to NCM and HADM, CCM and DADM showed a gradual and significantly higher release of GDF-5 in the second phase. Several burst releases of BMP-2 were characteristic for all matrices. The efficient adsorption and sustained protein release in the first 13 days, and the kinetics seen for HADM, with a burst release within hours and high amount of released growth factor within a secondary phase, may be beneficial for the long-term tissue regeneration following reconstructive periodontal surgery. View Full-Text
Keywords: biomaterials; xenografts; connective tissue grafts; bone and soft tissue regeneration; growth factors biomaterials; xenografts; connective tissue grafts; bone and soft tissue regeneration; growth factors
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MDPI and ACS Style

Nica, C.; Lin, Z.; Sculean, A.; Asparuhova, M.B. Adsorption and Release of Growth Factors from Four Different Porcine-Derived Collagen Matrices. Materials 2020, 13, 2635. https://doi.org/10.3390/ma13112635

AMA Style

Nica C, Lin Z, Sculean A, Asparuhova MB. Adsorption and Release of Growth Factors from Four Different Porcine-Derived Collagen Matrices. Materials. 2020; 13(11):2635. https://doi.org/10.3390/ma13112635

Chicago/Turabian Style

Nica, Cristina, Zhikai Lin, Anton Sculean, and Maria B. Asparuhova 2020. "Adsorption and Release of Growth Factors from Four Different Porcine-Derived Collagen Matrices" Materials 13, no. 11: 2635. https://doi.org/10.3390/ma13112635

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