Next Article in Journal
Numerical Assessment on Rotation Effect of the Stagnation Surface on Nanoparticle Deposition in Flame Synthesis
Next Article in Special Issue
PMMA-Based Bone Cements and the Problem of Joint Arthroplasty Infections: Status and New Perspectives
Previous Article in Journal
Diffusive Steel Scrap Melting in Carbon-Saturated Hot Metal—Phenomenological Investigation at the Solid–Liquid Interface
Previous Article in Special Issue
Influence of Porous Dressings Based on Butyric-Acetic Chitin Co-Polymer on Biological Processes In Vitro and In Vivo
Open AccessArticle

Cell-Free Demineralized Bone Matrix for Mesenchymal Stem Cells Survival and Colonization

1
Dipartimento di Scienze Cliniche e Molecolari-DISCLIMO, Università Politecnica delle Marche, Via Tronto 10/A, 60126 Ancona, Italy
2
Centro di Ricerca “E. Piaggio”, Università di Pisa, Via Diotisalvi 1, 56122 Pisa, Italy
3
Dipartimento di Scienza Applicata e Tecnologia—DISAT, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy
4
National Institute of Gastroenterology "S. de Bellis", Institute of Research, 70013 Castellana Grotte (BA), Italy
5
Dipartimento di Ingegneria dell’Informazione-DII, Università di Pisa, Via Caruso 16, 56122 Pisa, Italy
*
Author to whom correspondence should be addressed.
Materials 2019, 12(9), 1360; https://doi.org/10.3390/ma12091360
Received: 18 February 2019 / Revised: 9 April 2019 / Accepted: 24 April 2019 / Published: 26 April 2019
(This article belongs to the Special Issue Multifunctional Materials in Tissue Regeneration)
Decellularized bone matrix is receiving much attention as biological scaffolds and implantable biomaterials for bone tissue regeneration. Here, we evaluated the efficacy of a cell-free demineralized bone matrix on mesenchymal stem cells (MSCs) survival and differentiation in vitro. The seeding of human umbilical cord-derived MSCs (hUC-SCs) on decellularized bone matrices up to 14 days was exploited, assessing their capability of scaffold colonization and evaluating gene expression of bone markers. Light and Scanning Electron Microscopies were used. The obtained cell-free decalcified structures showed elastic moduli attributable to both topology and biochemical composition. Morphological observation evidenced an almost complete colonization of the scaffolds after 14 days of culture. Moreover, in hUC-SCs cultured on decalcified scaffolds, without the addition of any osteoinductive media, there was an upregulation of Collagen Type I (COL1) and osteonectin (ON) gene expression, especially on day 14. Modifications in the expression of genes engaged in stemness were also detected. In conclusion, the proposed decellularized bone matrix can induce the in vitro hUC-SCs differentiation and has the potential to be tested for in in vivo tissue regeneration. View Full-Text
Keywords: decellularized bone matrix; MSCs; gene expression; tissue engineering decellularized bone matrix; MSCs; gene expression; tissue engineering
Show Figures

Figure 1

MDPI and ACS Style

Mattioli-Belmonte, M.; Montemurro, F.; Licini, C.; Iezzi, I.; Dicarlo, M.; Cerqueni, G.; Coro, F.; Vozzi, G. Cell-Free Demineralized Bone Matrix for Mesenchymal Stem Cells Survival and Colonization. Materials 2019, 12, 1360.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop