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Materials 2019, 12(5), 840;

In Vitro Assessment of the Functional Dynamics of Titanium with Surface Coating of Hydroxyapatite Nanoparticles

Department of Rehabilitation, Federal Fluminense University, Rio de Janeiro 20000-000, Brazil
Oral Rehabilitation Carioca Centre, Campo Grande 79000-000, Brazil
Department of Oral Surgery and Implantology, Universidad Católica de Murcia, 30107 Murcia, Spain
Department of Dental Research, Universidad Federico Henriquez y Carvajal (UFHEC), Santo Domingo 10107, Dominican Republic
Molecular Interactions Laboratory, Facultad de Ciencias, Universidad de la República, Iguá 4225, Montevideo 11400, Uruguay
Department of Biotechnology, Biotecnos-Technology and Science, Cuareim 1483, Montevideo 11100, Uruguay
Biotechnology Department, Catholic University of Murcia (UCAM), 30107 Murcia, Spain
Author to whom correspondence should be addressed.
Received: 16 January 2019 / Revised: 7 March 2019 / Accepted: 8 March 2019 / Published: 12 March 2019
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Manipulation of implant surface characteristics constitutes a promising strategy for improving cell growth and tissue response on a variety of materials with different surface topographies. Mesenchymal progenitor cells with a capacity to respond to titanium surface stimuli and differentiate into osteoblasts were used to perform comparative tests between two different implant topographies, including their functional interaction with pre-osteoblasts directly seeded onto the implants. Functional analysis of nanostructured implant surfaces was performed by in vitro assay analysis. The machined surface of titanium implants (mach group) was used as a control and compared with a nanoparticle HA activated surface implant (nano group), developed by the deposition of pure crystalline hydroxyapatite. Cell culture on the nano group surface resulted in higher cell adhesion and cultured osteoblast viability compared with the mach group. Scanning electron microscope (SEM) images revealed a stable interaction, indicated by the presence of focal cell adhesion formation. These results together with positive mineralization assays showed the nano group to be an excellent scaffold for bone-implant integration. View Full-Text
Keywords: implants surface; mesenchymal cells; titanium implants implants surface; mesenchymal cells; titanium implants

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de Lima Cavalcanti, J.H.; Matos, P.C.; Depes de Gouvêa, C.V.; Carvalho, W.; Calvo-Guirado, J.L.; Aragoneses, J.M.; Pérez-Díaz, L.; Gehrke, S.A. In Vitro Assessment of the Functional Dynamics of Titanium with Surface Coating of Hydroxyapatite Nanoparticles. Materials 2019, 12, 840.

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