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Open AccessArticle

Balancing Purification and Ultrastructure of Naturally Derived Bone Blocks for Bone Regeneration: Report of the Purification Effort of Two Bone Blocks

1
Department of Oral and Maxillofacial Surgery, Working Group Biomaterials/Surfaces, University Hospital Hamburg-Eppendorf, Hamburg 20246, Germany; [email protected]@com (O.J.)
2
BerlinAnalytix GmbH, Berlin 12109, Germany
3
NMI, Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen 72770, Germany
4
Faculty of Applied Chemistry, Reutlingen University, Reutlingen 72770, Germany
5
Department for Cell and Tissue Engineering and Department of Biology and Human Genetics, Faculty of Medicine, University of Niš, 18100 Niš, Serbia
6
Department for Cell and Tissue Engineering, Faculty of Medicine, University of Niš, Niš 18100, Serbia
7
Department of Materials Test Engineering (WPT), TU Dortmund University, Dortmund 44227, Germany
*
Author to whom correspondence should be addressed.
Materials 2019, 12(19), 3234; https://doi.org/10.3390/ma12193234
Received: 2 September 2019 / Revised: 18 September 2019 / Accepted: 29 September 2019 / Published: 2 October 2019
(This article belongs to the Special Issue Bone Biomaterials)
The present publication reports the purification effort of two natural bone blocks, that is, an allogeneic bone block (maxgraft®, botiss biomaterials GmbH, Zossen, Germany) and a xenogeneic block (SMARTBONE®, IBI S.A., Mezzovico-Vira, Switzerland) in addition to previously published results based on histology. Furthermore, specialized scanning electron microscopy (SEM) and in vitro analyses (XTT, BrdU, LDH) for testing of the cytocompatibility based on ISO 10993-5/-12 have been conducted. The microscopic analyses showed that both bone blocks possess a trabecular structure with a lamellar subarrangement. In the case of the xenogeneic bone block, only minor remnants of collagenous structures were found, while in contrast high amounts of collagen were found associated with the allogeneic bone matrix. Furthermore, only island-like remnants of the polymer coating in case of the xenogeneic bone substitute seemed to be detectable. Finally, no remaining cells or cellular remnants were found in both bone blocks. The in vitro analyses showed that both bone blocks are biocompatible. Altogether, the purification level of both bone blocks seems to be favorable for bone tissue regeneration without the risk for inflammatory responses or graft rejection. Moreover, the analysis of the maxgraft® bone block showed that the underlying purification process allows for preserving not only the calcified bone matrix but also high amounts of the intertrabecular collagen matrix. View Full-Text
Keywords: bone block; allogeneic; xenogeneic; purification; bone regeneration; dentistry bone block; allogeneic; xenogeneic; purification; bone regeneration; dentistry
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MDPI and ACS Style

Barbeck, M.; Jung, O.; Xiong, X.; Krastev, R.; Korzinskas, T.; Najman, S.; Radenković, M.; Wegner, N.; Knyazeva, M.; Walther, F. Balancing Purification and Ultrastructure of Naturally Derived Bone Blocks for Bone Regeneration: Report of the Purification Effort of Two Bone Blocks. Materials 2019, 12, 3234. https://doi.org/10.3390/ma12193234

AMA Style

Barbeck M, Jung O, Xiong X, Krastev R, Korzinskas T, Najman S, Radenković M, Wegner N, Knyazeva M, Walther F. Balancing Purification and Ultrastructure of Naturally Derived Bone Blocks for Bone Regeneration: Report of the Purification Effort of Two Bone Blocks. Materials. 2019; 12(19):3234. https://doi.org/10.3390/ma12193234

Chicago/Turabian Style

Barbeck, Mike; Jung, Ole; Xiong, Xin; Krastev, Rumen; Korzinskas, Tadas; Najman, Stevo; Radenković, Milena; Wegner, Nils; Knyazeva, Marina; Walther, Frank. 2019. "Balancing Purification and Ultrastructure of Naturally Derived Bone Blocks for Bone Regeneration: Report of the Purification Effort of Two Bone Blocks" Materials 12, no. 19: 3234. https://doi.org/10.3390/ma12193234

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