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Article

PDMS-PMOXA-Nanoparticles Featuring a Cathepsin B-Triggered Release Mechanism

Department Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50, 4056 Basel, Switzerland
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Author to whom correspondence should be addressed.
Materials 2019, 12(17), 2836; https://doi.org/10.3390/ma12172836
Received: 2 July 2019 / Revised: 27 August 2019 / Accepted: 30 August 2019 / Published: 3 September 2019
Background: It was our intention to develop cathepsin B-sensitive nanoparticles for tumor-site-directed release. These nanoparticles should be able to release their payload as close to the tumor site with a decrease of off-target effects in mind. Cathepsin B, a lysosomal cysteine protease, is associated with premalignant lesions and invasive stages of cancer. Previous studies have shown cathepsin B in lysosomes and in the extracellular matrix. Therefore, this enzyme qualifies as a trigger for such an approach. Methods: Poly(dimethylsiloxane)-b-poly(methyloxazoline) (PDMS-PMOXA) nanoparticles loaded with paclitaxel were formed by a thin-film technique and standard coupling reactions were used for surface modifications. Despite the controlled release mechanism, the physical properties of the herein created nanoparticles were described. To characterize potential in vitro model systems, quantitative polymerase chain reaction and common bioanalytical methods were employed. Conclusions: Stable paclitaxel-loaded nanoparticles with cathepsin B digestible peptide were formed and tested on the ovarian cancer cell line OVCAR-3. These nanoparticles exerted a pharmacological effect on the tumor cells suggesting a release of the payload. View Full-Text
Keywords: enzyme-triggered-release; cathepsin B; paclitaxel; nanoparticles; PDMS-PMOXA; cancer; ovarian cancer enzyme-triggered-release; cathepsin B; paclitaxel; nanoparticles; PDMS-PMOXA; cancer; ovarian cancer
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MDPI and ACS Style

Ehrsam, D.; Porta, F.; Hussner, J.; Seibert, I.; Meyer zu Schwabedissen, H.E. PDMS-PMOXA-Nanoparticles Featuring a Cathepsin B-Triggered Release Mechanism. Materials 2019, 12, 2836. https://doi.org/10.3390/ma12172836

AMA Style

Ehrsam D, Porta F, Hussner J, Seibert I, Meyer zu Schwabedissen HE. PDMS-PMOXA-Nanoparticles Featuring a Cathepsin B-Triggered Release Mechanism. Materials. 2019; 12(17):2836. https://doi.org/10.3390/ma12172836

Chicago/Turabian Style

Ehrsam, Daniel, Fabiola Porta, Janine Hussner, Isabell Seibert, and Henriette E. Meyer zu Schwabedissen 2019. "PDMS-PMOXA-Nanoparticles Featuring a Cathepsin B-Triggered Release Mechanism" Materials 12, no. 17: 2836. https://doi.org/10.3390/ma12172836

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