Fabrication and Characterization of Low Methoxyl Pectin/Gelatin/Carboxymethyl Cellulose Absorbent Hydrogel Film for Wound Dressing Applications
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Reagents Used
2.2. Preparation of Hydrogel Films
2.3. Hydrogel Film Characterizations
2.3.1. Morphological Analysis Using Scanning Electron Microscopy
2.3.2. Film Thickness
2.3.3. Tensile Strength
2.3.4. Fluid Uptake Ability
2.3.5. Water Retention Capacity
2.3.6. Water Vapor Transmission Rate
2.3.7. Integrity Value
2.4. Test of Antibiotic-Containing Hydrogel Films’ Ability to Confer an Antimicrobial Property
2.5. Statistical Analysis
3. Results and Discussion
3.1. Preparation and Morphology of Hydrogel Films
3.2. Mechanical Properties of Hydrogel Films
3.3. Fluid Uptake Ability, Water Retention Capacity, Water Vapor Transmission Rate, and Integrity Value of Hydrogel Films
3.4. Anti-Bacterial Activity of Hydrogel Film Loaded with Povidone Iodine
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Code | LMP (%) | Gelatin (%) | CMC (%) | Glycerin (%) | Povidone Iodine | Crosslinking Agent | |
---|---|---|---|---|---|---|---|
Glu | CaCl2 | ||||||
F-Glu | 1 | 1 | 1 | - | - | + | - |
F-Glu-Ca | 1 | 1 | 1 | - | - | + | + |
F-Glu-Ca-G10 | 1 | 1 | 1 | 10 | - | + | + |
F-Glu-Ca-G20 | 1 | 1 | 1 | 20 | - | + | + |
F-Glu-Ca-G30 | 1 | 1 | 1 | 30 | - | + | + |
F-Glu-Ca-G40 | 1 | 1 | 1 | 40 | - | + | + |
F-Glu-Ca-G30-PI | 1 | 1 | 1 | 30 | 10 | + | + |
Hydrogel Film | Thickness (mm) | Tensile Strength (MPa) | Elongation at Break (%) | Young’s Modulus (N/cm2) |
---|---|---|---|---|
F-Glu | 0.30 ± 0.07 a | 715.73 ± 33.99 a | 9.43 ± 0.26 a | 8814.52 ± 163.07 a |
F-Glu-Ca | 0.35 ± 0.05 a | 853.06 ± 52.49 b | 3.22 ± 0.24 b | 56,781.02 ± 813.06 b |
F-Glu-Ca-G10 | 0.33 ± 0.04 a | 730.01 ± 98.11 a | 23.82 ± 2.91 c | 6494.36 ± 366.63 c |
F-Glu-Ca-G20 | 0.34 ± 0.03 a | 718.39 ± 41.97 a | 27.07 ± 1.96 c | 5707.38 ± 220.05 c |
F-Glu-Ca-G30 | 0.37 ± 0.06 a | 547.64 ± 77.53 c | 32.80 ± 1.14 d | 2926.71 ± 146.18 d |
F-Glu-Ca-G40 | 0.37 ± 0.09 a | 597.16 ± 31.13 c | 36.25 ± 4.47 d | 2835.37 ± 203.49 d |
Test Sample | Diameter of Inhibition Zone (mm) |
---|---|
10% Iodine solution F-Glu-Ca-G30-PI F-Glu-Ca-G30 | 99.23 ± 0.71 a 22.06 ± 3.44 b ND c |
DMSO | ND c |
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Jantrawut, P.; Bunrueangtha, J.; Suerthong, J.; Kantrong, N. Fabrication and Characterization of Low Methoxyl Pectin/Gelatin/Carboxymethyl Cellulose Absorbent Hydrogel Film for Wound Dressing Applications. Materials 2019, 12, 1628. https://doi.org/10.3390/ma12101628
Jantrawut P, Bunrueangtha J, Suerthong J, Kantrong N. Fabrication and Characterization of Low Methoxyl Pectin/Gelatin/Carboxymethyl Cellulose Absorbent Hydrogel Film for Wound Dressing Applications. Materials. 2019; 12(10):1628. https://doi.org/10.3390/ma12101628
Chicago/Turabian StyleJantrawut, Pensak, Juthamart Bunrueangtha, Juthamart Suerthong, and Nutthapong Kantrong. 2019. "Fabrication and Characterization of Low Methoxyl Pectin/Gelatin/Carboxymethyl Cellulose Absorbent Hydrogel Film for Wound Dressing Applications" Materials 12, no. 10: 1628. https://doi.org/10.3390/ma12101628