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Article

Assessment of Migration of Human MSCs through Fibrin Hydrogels as a Tool for Formulation Optimisation

1
School of Medicine Medical Sciences and Nutrition, University of Aberdeen, King’s College, Aberdeen AB24 3FX, UK
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Department of Materials Science and Metallurgy, University of Cambridge, 27 Charles Babbage Road, Cambridge CB3 0FS, UK
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School of Engineering, Newcastle University, Newcastle upon Tyne NE1 7RU, UK
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Materials and Engineering Research Institute, Sheffield Hallam University, City Campus, Howard Street, Sheffield S1 1WB, UK
*
Author to whom correspondence should be addressed.
Materials 2018, 11(9), 1781; https://doi.org/10.3390/ma11091781
Received: 30 July 2018 / Revised: 15 September 2018 / Accepted: 17 September 2018 / Published: 19 September 2018
(This article belongs to the Special Issue Scaffold Materials for Tissue Engineering)
Control of cell migration is fundamental to the performance of materials for cell delivery, as for cells to provide any therapeutic effect, they must migrate out from the delivery material. Here the influence of fibrinogen concentration on the migration of encapsulated human mesenchymal stem cells (hMSCs) from a cell spheroid through fibrin hydrogels is tracked over time. Fibrin was chosen as a model material as it is routinely employed as a haemostatic agent and more recently has been applied as a localised delivery vehicle for potential therapeutic cell populations. The hydrogels consisted of 5 U/mL thrombin and between 5 and 50 mg/mL fibrinogen. Microstructural and viscoelastic properties of different compositions were evaluated using SEM and rheometry. Increasing the fibrinogen concentration resulted in a visibly denser matrix with smaller pores and higher stiffness. hMSCs dispersed within the fibrin gels maintained cell viability post-encapsulation, however, the migration of cells from an encapsulated spheroid revealed that denser fibrin matrices inhibit cell migration. This study provides the first quantitative study on the influence of fibrinogen concentration on 3D hMSC migration within fibrin gels, which can be used to guide material selection for scaffold design in tissue engineering and for the clinical application of fibrin sealants. View Full-Text
Keywords: fibrin hydrogels; 3D cell migration; stem cells; cell delivery; regenerative medicine fibrin hydrogels; 3D cell migration; stem cells; cell delivery; regenerative medicine
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MDPI and ACS Style

Salam, N.; Toumpaniari, S.; Gentile, P.; Marina Ferreira, A.; Dalgarno, K.; Partridge, S. Assessment of Migration of Human MSCs through Fibrin Hydrogels as a Tool for Formulation Optimisation. Materials 2018, 11, 1781. https://doi.org/10.3390/ma11091781

AMA Style

Salam N, Toumpaniari S, Gentile P, Marina Ferreira A, Dalgarno K, Partridge S. Assessment of Migration of Human MSCs through Fibrin Hydrogels as a Tool for Formulation Optimisation. Materials. 2018; 11(9):1781. https://doi.org/10.3390/ma11091781

Chicago/Turabian Style

Salam, Nasseem, Sotiria Toumpaniari, Piergiorgio Gentile, Ana Marina Ferreira, Kenneth Dalgarno, and Simon Partridge. 2018. "Assessment of Migration of Human MSCs through Fibrin Hydrogels as a Tool for Formulation Optimisation" Materials 11, no. 9: 1781. https://doi.org/10.3390/ma11091781

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