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Open AccessArticle

Cell Type Influences Local Delivery of Biomolecules from a Bioinspired Apatite Drug Delivery System

1
Department of Biomedical Engineering, University of Connecticut Health Center, Farmington, CT 06030, USA
2
Department of Surgery, University of Connecticut Health, Farmington, CT 06030, USA
3
French National Institute of Health and Medical Research (INSERM), UMR 1260, Faculté de Médecine, University of Strasbourg, 67085 Strasbourg, France
4
Department of Medicine, University of Connecticut Health, Farmington, CT 06030, USA
*
Author to whom correspondence should be addressed.
Materials 2018, 11(9), 1703; https://doi.org/10.3390/ma11091703
Received: 10 July 2018 / Revised: 4 September 2018 / Accepted: 7 September 2018 / Published: 13 September 2018
(This article belongs to the Special Issue Bone Substitute Materials)
Recently, the benefit of step-wise sequential delivery of fibroblast growth factor-2 (FGF-2) and bone morphogenetic protein-2 from a bioinspired apatite drug delivery system on mouse calvarial bone repair was demonstrated. The thicknesses of the nanostructured poly-l-Lysine/poly-l-Glutamic acid polyelectrolyte multilayer (PEM) and the bone-like apatite barrier layer that make up the delivery system, were varied. The effects of the structural variations of the coating on the kinetics of cell access to a cytotoxic factor delivered by the layered structure were evaluated. FGF-2 was adsorbed into the outer PEM, and cytotoxic antimycin-A (AntiA) was adsorbed to the substrate below the barrier layer to detect the timing of the cell access. While MC3T3-E1 osteoprogenitor cells accessed AntiA after three days, the RAW 264.7 macrophage access occurred within 4 h, unless the PEM layer was removed, in which case the results were reversed. Pits were created in the coating by the RAW 264.7 macrophages and initiated delivery, while the osteoprogenitor cell access to drugs occurred through a solution-mediated coating dissolution, at junctions between the islands of crystals. Macrophage-mediated degradation is therefore a mechanism that controls drug release from coatings containing bioinspired apatite. View Full-Text
Keywords: biomimetic calcium phosphate; bone like apatite; polyelectrolyte multilayers; drug delivery; cell-biomaterial interactions biomimetic calcium phosphate; bone like apatite; polyelectrolyte multilayers; drug delivery; cell-biomaterial interactions
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Alhamdi, J.; Jacobs, E.; Gronowicz, G.; Benkirane-Jessel, N.; Hurley, M.; Kuhn, L. Cell Type Influences Local Delivery of Biomolecules from a Bioinspired Apatite Drug Delivery System. Materials 2018, 11, 1703.

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