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Open AccessFeature PaperArticle

Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella

1
National Research Institute of Electronics and Cryptology, The Scientific and Technological Research Council of Turkey (TUBITAK), Kocaeli 41470, Turkey
2
Turkey Public Health General Headquarter, Ankara 06100, Turkey
3
Institute of Chemistry, Technical University of Berlin, Straße des 17. Juni 124, Berlin 10623, Germany
*
Author to whom correspondence should be addressed.
Materials 2018, 11(9), 1541; https://doi.org/10.3390/ma11091541
Received: 12 August 2018 / Revised: 21 August 2018 / Accepted: 22 August 2018 / Published: 27 August 2018
(This article belongs to the Special Issue Nanomaterials in Health Care Diagnostics)
Bacteria-related pathogenic diseases are one of the major health problems throughout the world. Salmonella is a genus of rod-shaped Gram-negative enterobacteria of which more than 2600 serotypes have been identified. Infection with Salmonella can cause salmonellosis, a serious bacterial toxi-infection syndrome associated with gastroenteritis, and paralyphoid and typhoid fevers. Its rapid and sensitive detection is a key to the prevention of problems related to health. This paper describes the development of antibody and DNA sensors for Salmonella detection using a microfluidic-based electrochemical system. Commercial Salmonella typhimurium and Salmonella typhimurium from human stool samples were investigated using standard and nanomaterial-amplified antibody sensors. S. typhimurium could be detected down to 1 cfu mL−1. The specificity of immunoassay was tested by studying with non-specific bacteria including E. coli and S. aureus that revealed only 2.01% and 2.66% binding when compared to the target bacterium. On the other hand, the quantification of Salmonella DNA was investigated in a concentration range of 0.002–200 µM using the developed DNA biosensor that demonstrated very high specificity and sensitivity with a detection limit of 0.94 nM. Our custom-designed microfluidic sensor offers rapid, highly sensitive, and specific diagnostic assay approaches for pathogen detection. View Full-Text
Keywords: Salmonella spp.; pathogen detection; antibody biosensor; DNA biosensor; microfluidic-based electrochemical sensor; nanoparticle enhanced bio-detection; infectious diseases Salmonella spp.; pathogen detection; antibody biosensor; DNA biosensor; microfluidic-based electrochemical sensor; nanoparticle enhanced bio-detection; infectious diseases
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MDPI and ACS Style

Savas, S.; Ersoy, A.; Gulmez, Y.; Kilic, S.; Levent, B.; Altintas, Z. Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella. Materials 2018, 11, 1541.

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