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Open AccessFeature PaperArticle

Osteogenic Potential of Pre-Osteoblastic Cells on a Chitosan-graft-Polycaprolactone Copolymer

1
Department of Materials Science and Technology, University of Crete, Voutes Campus, 70013 Heraklion, Greece
2
Institute of Electronic Structure and Laser, Foundation for Research and Technology-Hellas, Nikolaou Plastira 100, 70013 Heraklion, Greece
*
Author to whom correspondence should be addressed.
Materials 2018, 11(4), 490; https://doi.org/10.3390/ma11040490
Received: 5 February 2018 / Revised: 15 March 2018 / Accepted: 21 March 2018 / Published: 26 March 2018
(This article belongs to the Special Issue Selected papers from EUROMAT 2017 Conference—Biomaterials)
A chitosan-graft-polycaprolactone (CS-g-PCL) copolymer synthesized via a multi-step process was evaluated as a potential biomaterial for the adhesion and growth of MC3T3-E1 pre-osteoblastic cells. A strong adhesion of the MC3T3-E1 cells with a characteristic spindle-shaped morphology was observed from the first days of cell culture onto the copolymer surfaces. The viability and proliferation of the cells on the CS-g-PCL surfaces, after 3 and 7 days in culture, were significantly higher compared to the cells cultured on the tissue culture treated polystyrene (TCPS) control. The osteogenic potential of the pre-osteoblastic cells cultured on CS-g-PCL surfaces was evaluated by determining various osteogenic differentiation markers and was compared to the TCPS control surface. Specifically, alkaline phosphatase activity levels show significantly higher values at both time points compared to TCPS, while secreted collagen into the extracellular matrix was found to be higher on day 7. Calcium biomineralization deposited into the matrix is significantly higher for the CS-g-PCL copolymer after 14 days in culture, while the levels of intracellular osteopontin were significantly higher on the CS-g-PCL surfaces compared to TCPS. The enhanced osteogenic response of the MC3T3-E1 pre-osteoblasts cultured on CS-g-PCL reveals that the copolymer underpins the cell functions towards bone tissue formation and is thus an attractive candidate for use in bone tissue engineering. View Full-Text
Keywords: biomaterial; chitosan; polycaprolactone; osteogenic potential; bone tissue engineering; pre-osteoblastic cells biomaterial; chitosan; polycaprolactone; osteogenic potential; bone tissue engineering; pre-osteoblastic cells
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Georgopoulou, A.; Kaliva, M.; Vamvakaki, M.; Chatzinikolaidou, M. Osteogenic Potential of Pre-Osteoblastic Cells on a Chitosan-graft-Polycaprolactone Copolymer. Materials 2018, 11, 490.

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