Chitosan/Poly(vinylpyrrolidone) Matrices Obtained by Gamma-Irradiation for Skin Scaffolds: Characterization and Preliminary Cell Response Studies
by
Maria Helena Casimiro 1,*
, Susana R. Gomes 1, Gabriela Rodrigues 2, João Paulo Leal 1,3 and Luís M. Ferreira 1
Maria Helena Casimiro 1,*, Susana R. Gomes 1, Gabriela Rodrigues 2, João Paulo Leal 1,3 and Luís M. Ferreira 1
1
Centro de Ciências e Tecnologias Nucleares (C2TN), Instituto Superior Técnico, Universidade de Lisboa, Estrada Nacional 10, 2695-066 Bobadela, LRS, Portugal
2
Centro de Ecologia, Evolução e Alterações Ambientais (cE3c) and Departamento de Biologia Animal, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal
3
Centro de Química Estrutural (CQE), Instituto Superior Técnico, Estrada Nacional 10, 2695-066 Bobadela, LRS, Portugal
*
Author to whom correspondence should be addressed.
Materials 2018, 11(12), 2535; https://doi.org/10.3390/ma11122535
Received: 31 October 2018 / Revised: 22 November 2018 / Accepted: 6 December 2018 / Published: 13 December 2018
(This article belongs to the Special Issue Scaffold Materials for Tissue Engineering)
Several studies have shown that chitosan possesses characteristics favorable for promoting dermal regeneration and accelerated wound healing. In this work we have reported the work that has been done on the development and characterization of biocompatible and biodegradable chitosan based matrices to be used as skin scaffolds. Poly(vinylpyrrrolidone) (PVP) was used as copolymer and a two steps methodology of freeze-drying and gamma irradiation was used to obtain the porous matrices. The influence of PVP content, synthesis procedure and absorbed radiation dose on matrices’ physical, chemical and structural properties was evaluated by ATR-FTIR, TGA, SEM, contact angle measurements and degradation behavior. The in vitro cellular viability and proliferation of HFFF2 fibroblast cell line was analyzed as a measure of matrices’ biocompatibility and ability to assist skin regeneration. Results show that over the studied range values, gamma-radiation dose, copolymer concentration and synthesis procedure can be used to tailor the matrices’ morphology in terms of porosity and surface roughness. Early results from biological assays evidence the biocompatibility of the prepared chitosan/PVP matrices since cells adhered to the surface of all matrices (chitosan/PVP (5%) γ-irradiated at 10 kGy presents the higher cellular viability). These features show that the resultant matrices could be a potential suitable scaffold for skin tissue regeneration.
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MDPI and ACS Style
Casimiro, M.H.; Gomes, S.R.; Rodrigues, G.; Leal, J.P.; Ferreira, L.M. Chitosan/Poly(vinylpyrrolidone) Matrices Obtained by Gamma-Irradiation for Skin Scaffolds: Characterization and Preliminary Cell Response Studies. Materials 2018, 11, 2535.
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