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Materials 2018, 11(1), 68; https://doi.org/10.3390/ma11010068

Uptake of Tyrosine Amino Acid on Nano-Graphene Oxide

1
Chemistry Department, Faculty of Science at Yanbu, Taibah University, Yanbu 46423, Saudi Arabia
2
Chemistry Department, Faculty of Science, Damietta University, Damietta 34517, Egypt
3
Chemistry Department, Faculty of Science, Alexandria University, Alexandria 23132, Egypt
4
Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City 32897, Egypt
*
Author to whom correspondence should be addressed.
Received: 3 December 2017 / Revised: 26 December 2017 / Accepted: 29 December 2017 / Published: 4 January 2018
(This article belongs to the Section Carbon Materials)
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Abstract

Graphene oxide (GO) is emerging as a promising nanomaterial with potential application in the detection and analysis of amino acids, DNA, enzymes, and proteins in biological fluid samples. So, the reaction of GO with amino acids should be characterized and determined before using it in biosensing methods and devices. In this study, the reaction of tyrosine amino acid (Tyr) with GO was characterized using FT-IR, UV-vis spectrophotometry, and scanning electron microscopy (SEM) before its use. The optimum conditions for GO’s interaction with Tyr amino acid have been studied under variable conditions. The optimum conditions of pH, temperature, shaking time, and GO and tyrosine concentrations for the uptaking of tyrosine amino acid onto the GO’s surface from aqueous solution were determined. The SEM analysis showed that the GO supplied was in a particle size range between 5.4 and 8.1 nm. A pH of 8.4–9.4 at 25 °C and 5 min of shaking time were the optimum conditions for a maximum uptake of 1.4 μg/mL of tyrosine amino acid onto 0.2 mg/mL of GO. View Full-Text
Keywords: graphene oxide; amino acid; tyrosine; uptaking; SEM graphene oxide; amino acid; tyrosine; uptaking; SEM
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Nassef, H.M.; Hagar, M.; Malek, Z.; Othman, A.M. Uptake of Tyrosine Amino Acid on Nano-Graphene Oxide. Materials 2018, 11, 68.

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