Histological and Immunohistochemical Analyses of Repair of the Disc in the Rabbit Temporomandibular Joint Using a Collagen Template

Wang, Kuo-Hwa; Chan, Wing P.; Chiu, Li-Hsuan; Tsai, Yu-Hui; Fang, Chia-Lang; Yang, Charn-Bing; Chen, Kuan-Chou; Tsai, Hung-Li; Lai, Wen-Fu

doi:10.3390/ma10080924

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Histological and Immunohistochemical Analyses of Repair of the Disc in the Rabbit Temporomandibular Joint Using a Collagen Template

^1,†,

^2,†,

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⁴,

⁵,

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^4,‡ and

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Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan

Department of Radiology, School of Medicine, College of Medicine, Taipei Medical Univesity, Taipei 110, Taiwan

McLean Imaging Center, Harvard Medical School, Belmont, MA 02478, USA

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Graduate Institute of Medical Sciences, Taipei Medical University, Taipei 11031, Taiwan

⁵

Department of Pathology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan

⁶

School of Dentistry, College of Dentistry, Taipei Medical University, Taipei 11031, Taiwan

Author to whom correspondence should be addressed.

^†

Co-first authors contributed equally to this article.

^‡

Co-corresponding author.

Materials 2017, 10(8), 924; https://doi.org/10.3390/ma10080924

Received: 7 July 2017 / Revised: 31 July 2017 / Accepted: 3 August 2017 / Published: 9 August 2017

(This article belongs to the Section Biomaterials)

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Abstract

A previous study demonstrated that the reconstituted type I collagen matrix extracted from rabbit tendons enabled the TMJ disc to regenerate in the rabbit. The aim of this study was to investigate changes in the extracellular matrix (ECM) and mechanisms of regeneration in the TMJ disc. In 36 New Zealand rabbits that underwent a partial discectomy, discs were replaced with reconstituted collagen templates for 3 months. A histological analysis showed that moderate to severe degeneration appeared in partially discectomized joints without implantation. In contrast, discs experienced regeneration of reconstituted collagen template implantation and the joint returned to normal function. Cells in the regenerative tissue expressed ECM, and fibers became regular and compact due to tissue remodeling over time. Reparative cells differentiated into chondroblasts, and showed highly dense pericellular fibers. The morphology and collagen composition of the disc and condyle in the 3-month experimental group were similar to those of normal tissues. In conclusion, the reconstituted collagen template facilitated the regeneration of surgically discectomized discs. Type I and type II collagens play a crucial role in the regeneration of articular discs. View Full-Text

Keywords: temporomandibular joint disc; reconstituted collagen template; tissue regeneration

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MDPI and ACS Style

Wang, K.-H.; Chan, W.P.; Chiu, L.-H.; Tsai, Y.-H.; Fang, C.-L.; Yang, C.-B.; Chen, K.-C.; Tsai, H.-L.; Lai, W.-F. Histological and Immunohistochemical Analyses of Repair of the Disc in the Rabbit Temporomandibular Joint Using a Collagen Template. Materials 2017, 10, 924. https://doi.org/10.3390/ma10080924

AMA Style

Wang K-H, Chan WP, Chiu L-H, Tsai Y-H, Fang C-L, Yang C-B, Chen K-C, Tsai H-L, Lai W-F. Histological and Immunohistochemical Analyses of Repair of the Disc in the Rabbit Temporomandibular Joint Using a Collagen Template. Materials. 2017; 10(8):924. https://doi.org/10.3390/ma10080924

Chicago/Turabian Style

Wang, Kuo-Hwa, Wing P. Chan, Li-Hsuan Chiu, Yu-Hui Tsai, Chia-Lang Fang, Charn-Bing Yang, Kuan-Chou Chen, Hung-Li Tsai, and Wen-Fu Lai. 2017. "Histological and Immunohistochemical Analyses of Repair of the Disc in the Rabbit Temporomandibular Joint Using a Collagen Template" Materials 10, no. 8: 924. https://doi.org/10.3390/ma10080924

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Histological and Immunohistochemical Analyses of Repair of the Disc in the Rabbit Temporomandibular Joint Using a Collagen Template

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