Next Article in Journal
Effect of Preservative Pretreatment on the Biological Durability of Corn Straw Fiber/HDPE Composites
Next Article in Special Issue
Characterisation of Asphalt Concrete Using Nanoindentation
Previous Article in Journal
An Intelligent Gear Fault Diagnosis Methodology Using a Complex Wavelet Enhanced Convolutional Neural Network
Previous Article in Special Issue
Full-Field Indentation Damage Measurement Using Digital Image Correlation
Article Menu
Issue 7 (July) cover image

Export Article

Open AccessArticle
Materials 2017, 10(7), 788; https://doi.org/10.3390/ma10070788

Using Biotechnology to Solve Engineering Problems: Non-Destructive Testing of Microfabrication Components

1
iBB-Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal
2
UNIDEMI, Department of Mechanical and Industrial Engineering, NOVA School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal
*
Author to whom correspondence should be addressed.
Received: 31 May 2017 / Revised: 4 July 2017 / Accepted: 8 July 2017 / Published: 12 July 2017
(This article belongs to the Special Issue Advanced Nanoindentation in Materials)
Full-Text   |   PDF [4214 KB, uploaded 12 July 2017]   |  

Abstract

In an increasingly miniaturised technological world, non-destructive testing (NDT) methodologies able to detect defects at the micro scale are necessary to prevent failures. Although several existing methods allow the detection of defects at that scale, their application may be hindered by the small size of the samples to examine. In this study, the application of bacterial cells to help the detection of fissures, cracks, and voids on the surface of metals is proposed. The application of magnetic and electric fields after deposition of the cells ensured the distribution of the cells over the entire surfaces and helped the penetration of the cells inside the defects. The use of fluorophores to stain the cells allowed their visualisation and the identification of the defects. Furthermore, the size and zeta potential of the cells and their production of siderophores and biosurfactants could be influenced to detect smaller defects. Micro and nano surface defects made in aluminium, steel, and copper alloys could be readily identified by two Staphylococcus strains and Rhodococcus erythropolis cells. View Full-Text
Keywords: micro defects; NDT; indentation; microfabrication; Staphylococcus; Rhodococcus micro defects; NDT; indentation; microfabrication; Staphylococcus; Rhodococcus
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

de Carvalho, C.C.C.R.; Inácio, P.L.; Miranda, R.M.; Santos, T.G. Using Biotechnology to Solve Engineering Problems: Non-Destructive Testing of Microfabrication Components. Materials 2017, 10, 788.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Materials EISSN 1996-1944 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top