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Materials 2017, 10(12), 1387; https://doi.org/10.3390/ma10121387

The Osteogenic and Tenogenic Differentiation Potential of C3H10T1/2 (Mesenchymal Stem Cell Model) Cultured on PCL/PLA Electrospun Scaffolds in the Absence of Specific Differentiation Medium

1
CNRS, UMR 7338 Biomechanics and Bioengineering, Sorbonne Universités, Université de Technologie de Compiègne, 60200 Compiègne, France
2
CNRS UMR 7622 IBPS-Developmental Biology Laboratory, Sorbonne Universités, UPMC Univ Paris 06, F-75005 Paris, France
3
Inserm U1156, F-75005 Paris, France
4
Institute for Multiphase Processes, Leibniz Universität Hanover, D-30167 Hanover, Germany
5
CNRS, UMR 7337 Roberval Laboratory for Mechanics, Sorbonne Universités, Université de Technologie de Compiègne, 60200 Compiègne, France
*
Author to whom correspondence should be addressed.
Received: 3 November 2017 / Revised: 27 November 2017 / Accepted: 29 November 2017 / Published: 4 December 2017
(This article belongs to the Special Issue Polymeric Materials for Medical Applications)
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Abstract

The differentiation potential of mesenchymal stem cells (MSC) has been extensively tested on electrospun scaffolds. However, this potential is often assessed with lineage-specific medium, making it difficult to interpret the real contribution of the properties of the scaffold in the cell response. In this study, we analyzed the ability of different polycaprolactone/polylactic acid PCL/PLA electrospun scaffolds (pure or blended compositions, random or aligned fibers, various fiber diameters) to drive MSC towards bone or tendon lineages in the absence of specific differentiation medium. C3H10T1/2 cells (a mesenchymal stem cell model) were cultured on scaffolds for 96 h without differentiation factors. We performed a cross-analysis of the cell–scaffold interactions (spreading, organization, and specific gene expression) with mechanical (elasticity), morphological (porosity, fibers diameter and orientation) and surface (wettability) characterizations of the electrospun fibers. We concluded that (1) osteogenic differentiation can be initiated on pure PCL-based electrospun scaffolds without specific culture conditions; (2) fiber alignment modified cell organization in the short term and (3) PLA added to PCL with an increased fiber diameter encouraged the stem cells towards the tendon lineage without additional tenogenic factors. In summary, the differentiation potential of stem cells on adapted electrospun fibers could be achieved in factor-free medium, making possible future applications in clinically relevant situations. View Full-Text
Keywords: scaffold; polymer; electrospinning; mesenchymal stem cell; cell differentiation; tissue engineering scaffold; polymer; electrospinning; mesenchymal stem cell; cell differentiation; tissue engineering
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Baudequin, T.; Gaut, L.; Mueller, M.; Huepkes, A.; Glasmacher, B.; Duprez, D.; Bedoui, F.; Legallais, C. The Osteogenic and Tenogenic Differentiation Potential of C3H10T1/2 (Mesenchymal Stem Cell Model) Cultured on PCL/PLA Electrospun Scaffolds in the Absence of Specific Differentiation Medium. Materials 2017, 10, 1387.

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