Novel Dental Adhesive with Biofilm-Regulating and Remineralization Capabilities
AbstractThe mechanical properties and anti-caries effect of a novel anti-caries adhesive containing poly (amidoamine) dendrimer (PAMAM) and dimethylaminododecyl methacrylate (DMADDM) were investigated for the first time. Microtensile bond strength and surface charge density were measured for the novel anti-caries adhesives. Streptococcus mutans, Streptococcus sanguinis, and Streptococcus gordonii were chosen to form three-species biofilms. Lactic acid assay, MTT (3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, exopolysaccharide staining and live/dead staining were performed to study anti-biofilm effect of the adhesive. The TaqMan realtime polymerase chain reaction was used to study the proportion change in three-species biofilms of different groups. The Scanning Electron Microscope (SEM) was used to observe the remineralization effect of PAMAM and DMADDM. The results showed that incorporating PAMAM and DMADDM into adhesive had no adverse effect on the dentin bond strength. The 1% PAMAM and 5% DMADDM adhesive group showed anti-biofilm properties and developed a healthier biofilm with a lower chance of inducing dental caries. Combination of 1% PAMAM and 5% DMADDM solution maintained remineralization capability on dentin, similar to that using 1% PAMAM alone. In conclusion, the adhesive containing PAMAM and DMADDM had strong antimicrobial properties and biological remineralization capabilities, and is promising for anti-caries clinical applications. View Full-Text
- Supplementary File 1:
Supplementary (PDF, 690 KB)
Share & Cite This Article
Ge, Y.; Ren, B.; Zhou, X.; Xu, H.H.K.; Wang, S.; Li, M.; Weir, M.D.; Feng, M.; Cheng, L. Novel Dental Adhesive with Biofilm-Regulating and Remineralization Capabilities. Materials 2017, 10, 26.
Ge Y, Ren B, Zhou X, Xu HHK, Wang S, Li M, Weir MD, Feng M, Cheng L. Novel Dental Adhesive with Biofilm-Regulating and Remineralization Capabilities. Materials. 2017; 10(1):26.Chicago/Turabian Style
Ge, Yang; Ren, Biao; Zhou, Xuedong; Xu, Hockin H.K.; Wang, Suping; Li, Mingyun; Weir, Michael D.; Feng, Mingye; Cheng, Lei. 2017. "Novel Dental Adhesive with Biofilm-Regulating and Remineralization Capabilities." Materials 10, no. 1: 26.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.