Elevated Microsatellite Alterations at Selected Tetranucleotide Repeats (EMAST) in Penile Squamous Cell Carcinoma—No Evidence for a Role in Carcinogenesis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients and Tissue Samples
2.2. Microdissection and DNA Isolation
2.3. EMAST Detection
2.4. Immunohistochemical Analysiys of MSH3
2.5. HPV PCR Analysis of Penile Tumors
3. Results
3.1. Detection of HPV in pSCC Cases
3.2. Immunohistochemical Evaluation of MSH3 Expression
3.3. Determination of EMAST Status
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cases (n = 78) | ||
---|---|---|
Age, years | ||
Median age | 68 | |
Mean age | 67.6 ± 11.6 | |
Range | 39–93 | |
Tumor Stage | n= | |
pTis | 7 | |
pT1a | 30 | |
pT1b | 5 | |
pT2 | 23 | |
pT3 | 11 | |
pT4 | 1 | |
unknown | 1 | |
Tumor Grade | n= | |
1 | 16 | |
2 | 38 | |
3 | 16 | |
Unknown | 1 | |
HPV Status | n= | |
Positive | 28 | |
Negative | 50 | |
Histological subtype | HPV negative (n) | HPV positive (n) |
Usual type | 31 | 3 |
Verrucous | 9 | - |
Basaloid | 3 | 11 |
Warty-basaloid | 1 | 6 |
Pseudohyperplastic | 3 | - |
Warty | 1 | 1 |
Lymphoepithelioma-like | - | 1 |
Clear cell | - | 1 |
Carcinoma cuniculatum | 1 | - |
Unknown: n = 1 |
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Fiegl, A.; Wendler, O.; Giedl, J.; Gaisa, N.T.; Richter, G.; Campean, V.; Burger, M.; Simmer, F.; Nagtegaal, I.; Wullich, B.; et al. Elevated Microsatellite Alterations at Selected Tetranucleotide Repeats (EMAST) in Penile Squamous Cell Carcinoma—No Evidence for a Role in Carcinogenesis. Curr. Oncol. 2024, 31, 5752-5761. https://doi.org/10.3390/curroncol31100427
Fiegl A, Wendler O, Giedl J, Gaisa NT, Richter G, Campean V, Burger M, Simmer F, Nagtegaal I, Wullich B, et al. Elevated Microsatellite Alterations at Selected Tetranucleotide Repeats (EMAST) in Penile Squamous Cell Carcinoma—No Evidence for a Role in Carcinogenesis. Current Oncology. 2024; 31(10):5752-5761. https://doi.org/10.3390/curroncol31100427
Chicago/Turabian StyleFiegl, August, Olaf Wendler, Johannes Giedl, Nadine T. Gaisa, Georg Richter, Valentina Campean, Maximilian Burger, Femke Simmer, Iris Nagtegaal, Bernd Wullich, and et al. 2024. "Elevated Microsatellite Alterations at Selected Tetranucleotide Repeats (EMAST) in Penile Squamous Cell Carcinoma—No Evidence for a Role in Carcinogenesis" Current Oncology 31, no. 10: 5752-5761. https://doi.org/10.3390/curroncol31100427