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Brief Report

Comprehensive Laboratory Diagnostic Workup for Patients with Suspected Intraocular Lymphoma including Flow Cytometry, Molecular Genetics and Cytopathology

1
Department of Hematology and Central Hematology Laboratory, Inselspital, Bern University Hospital, University of Bern, 3012 Bern, Switzerland
2
Department of Medicine A, Haematology, Oncology, and Pneumology, University Hospital Münster, 48149 Münster, Germany
3
Department of Hematology and Medical Oncology, University Medicine Göttingen (UMG), 37075 Göttingen, Germany
4
Department of Ophthalmology, Inselspital, Bern University Hospital, University of Bern, 3012 Bern, Switzerland
5
Department of Medical Oncology, Inselspital, Bern University Hospital, 3012 Bern, Switzerland
6
Institute of Pathology, University of Bern, 3012 Bern, Switzerland
*
Author to whom correspondence should be addressed.
These authors contributed equally and should be considered as first authors.
Curr. Oncol. 2022, 29(2), 766-776; https://doi.org/10.3390/curroncol29020065
Received: 24 December 2021 / Revised: 24 January 2022 / Accepted: 28 January 2022 / Published: 31 January 2022
(This article belongs to the Section Hematology)
Background: Intraocular lymphoma (IOL) presents a real challenge in daily diagnostics. Cyto- and/or histopathology of vitreous body represent the diagnostic cornerstones. Yet, false negative results remain common. Therefore, we analyzed the diagnostic significance of flow cytometry (FC) within the workup algorithm of IOL and compared its sensitivity with the results obtained from routine cytopathology and molecular genetics; Methods: Seven patients undergoing vitrectomy due to suspected IOL were investigated by FC and parallel cytopathology and, if available, digital droplet PCR (ddPCR) for MYD88 L265P; Results: Four out of seven patients were finally diagnosed with IOL. Among the IOL patients, cytopathology confirmed the presence of lymphoma cells in only two cases. In contrast, FC was positive for IOL in all four cases, and FC additionally confirmed the lack of IOL in the remaining patients. In IOL patients diagnosed by FC and with available ddPCR, the diagnosis of IOL was confirmed by the presence of the MYD88 L265P mutation in all three patients; Conclusions: The combination with FC was superior to cytopathology alone in the diagnostic work-up of IOL, and it showed an excellent correlation with ddPCR results. A comprehensive diagnostic panel consisting of cytopathology, FC and molecular genetics should be considered for the work-up of suspected IOL. View Full-Text
Keywords: intraocular lymphoma; vitreous body; flow cytometry; molecular genetics; MYD88; cytopathology intraocular lymphoma; vitreous body; flow cytometry; molecular genetics; MYD88; cytopathology
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MDPI and ACS Style

Shumilov, E.; Mazzeo, P.; Zinkernagel, M.S.; Legros, M.; Porret, N.; Romagna, L.; Haase, D.; Lenz, G.; Novak, U.; Banz, Y.; Pabst, T.; Bacher, U. Comprehensive Laboratory Diagnostic Workup for Patients with Suspected Intraocular Lymphoma including Flow Cytometry, Molecular Genetics and Cytopathology. Curr. Oncol. 2022, 29, 766-776. https://doi.org/10.3390/curroncol29020065

AMA Style

Shumilov E, Mazzeo P, Zinkernagel MS, Legros M, Porret N, Romagna L, Haase D, Lenz G, Novak U, Banz Y, Pabst T, Bacher U. Comprehensive Laboratory Diagnostic Workup for Patients with Suspected Intraocular Lymphoma including Flow Cytometry, Molecular Genetics and Cytopathology. Current Oncology. 2022; 29(2):766-776. https://doi.org/10.3390/curroncol29020065

Chicago/Turabian Style

Shumilov, Evgenii, Paolo Mazzeo, Martin S. Zinkernagel, Myriam Legros, Naomi Porret, Lorenz Romagna, Detlef Haase, Georg Lenz, Urban Novak, Yara Banz, Thomas Pabst, and Ulrike Bacher. 2022. "Comprehensive Laboratory Diagnostic Workup for Patients with Suspected Intraocular Lymphoma including Flow Cytometry, Molecular Genetics and Cytopathology" Current Oncology 29, no. 2: 766-776. https://doi.org/10.3390/curroncol29020065

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