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Article

Prospective Monitoring of Circulating Epithelial Tumor Cells (CETC) Reveals Changes in Gene Expression during Adjuvant Radiotherapy of Breast Cancer Patients

1
Department of Radiotherapy and Radiation Oncology, University Hospital Jena, Bachstraße 18, 07743 Jena, Germany
2
Transfusion Center Bayreuth, Kurpromenade 2, 95448 Bayreuth, Germany
*
Author to whom correspondence should be addressed.
Curr. Oncol. 2021, 28(5), 3507-3524; https://doi.org/10.3390/curroncol28050302
Received: 20 July 2021 / Revised: 22 August 2021 / Accepted: 3 September 2021 / Published: 8 September 2021
(This article belongs to the Special Issue Translational Research in Breast Cancer Patients)
Circulating epithelial tumor cells (CETC) are considered to be responsible for the formation of metastases. Therefore, their importance as prognostic and/or predictive markers in breast cancer is being intensively investigated. Here, the reliability of single cell expression analyses in isolated and collected CETC from whole blood samples of patients with early-stage breast cancer before and after radiotherapy (RT) using the maintrac® method was investigated. Single-cell expression analyses were performed with qRT-PCR on a panel of selected genes: GAPDH, EpCAM, NANOG, Bcl-2, TLR 4, COX-2, PIK3CA, Her-2/neu, Vimentin, c-Met, Ki-67. In all patients, viable CETC were detected prior to and at the end of radiotherapy. In 7 of the 9 (77.8%) subjects examined, the CETC number at the end of the radiotherapy series was higher than before. The majority of genes analyzed showed increased expression after completion of radiotherapy compared to baseline. Procedures and methods used in this pilot study proved to be feasible. The method is suitable for further investigation of the underlying molecular biological mechanisms occurring in cells surviving radiotherapy and possibly the development of radiation resistance. View Full-Text
Keywords: circulating tumor cells; gene expression analysis; radiotherapy; early-stage breast cancer; prognostic marker circulating tumor cells; gene expression analysis; radiotherapy; early-stage breast cancer; prognostic marker
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MDPI and ACS Style

Mäurer, M.; Pachmann, K.; Wendt, T.; Schott, D.; Wittig, A. Prospective Monitoring of Circulating Epithelial Tumor Cells (CETC) Reveals Changes in Gene Expression during Adjuvant Radiotherapy of Breast Cancer Patients. Curr. Oncol. 2021, 28, 3507-3524. https://doi.org/10.3390/curroncol28050302

AMA Style

Mäurer M, Pachmann K, Wendt T, Schott D, Wittig A. Prospective Monitoring of Circulating Epithelial Tumor Cells (CETC) Reveals Changes in Gene Expression during Adjuvant Radiotherapy of Breast Cancer Patients. Current Oncology. 2021; 28(5):3507-3524. https://doi.org/10.3390/curroncol28050302

Chicago/Turabian Style

Mäurer, Matthias, Katharina Pachmann, Thomas Wendt, Dorothea Schott, and Andrea Wittig. 2021. "Prospective Monitoring of Circulating Epithelial Tumor Cells (CETC) Reveals Changes in Gene Expression during Adjuvant Radiotherapy of Breast Cancer Patients" Current Oncology 28, no. 5: 3507-3524. https://doi.org/10.3390/curroncol28050302

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