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Current Oncology
Volume 23
Issue 2
10.3747/co.23.2830
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Current Oncology is published by MDPI from Volume 28 Issue 1 (2021). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Multimed Inc..
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Article

MicroRNA Expression Profiling of Sputum for the Detection of Early and Locally Advanced Non-Small-Cell Lung Cancer: A Prospective Case–Control Study

by
R. Razzak
1,*,
E.L.R. Bédard
1,
J.O. Kim
2,
S. Gazala
1,
L. Guo
3,
S. Ghosh
3,
A. Joy
3,
T. Nijjar
3,
E. Wong
3 and
W.H. Roa
4
1
Department of Surgery, Faculty of Medicine and Dentistry, University of Alberta, Office of Surgical Education, 2D2.01 Walter Mackenzie Health Sciences Centre, University of Alberta, 844–112 Street, Edmonton, Alberta T6G 2B7, Canada
2
CancerCare Manitoba, Dept Radiat Oncol, Winnipeg, MB, Canada
3
Cross Canc Inst, Dept Oncol, Edmonton, AB T6G 1Z2, Canada
4
Univ Alberta Hosp, Dept Internal Med, Div Pulm Med, Edmonton, AB T6G 2B7, Canada
*
Author to whom correspondence should be addressed.
Curr. Oncol. 2016, 23(2), 86-94; https://doi.org/10.3747/co.23.2830
Submission received: 2 January 2016 / Revised: 3 February 2016 / Accepted: 5 March 2016 / Published: 1 April 2016

Abstract

Background: Non-small-cell lung cancer (NSCLC) is associated with very poor overall survival because 70% of patients present with locally advanced or metastatic disease at the time of diagnosis. MicroRNAS (miRNAS) are a class of short, noncoding RNA molecules whose presence in samples of biologic fluids such as sputum has demonstrated promise as a potential means of detecting NSCLC. We investigated the stage-specific NSCLC detection potential of an efficient panel of 3 miRNAS (MIR-21, MIR-210, MIR-372) using a single sputum sample. Methods: A single spontaneously expectorated sputum sample was prospectively collected from 21 early NSCLC (≤stage II) patients, 22 advanced NSCLC (≥stage III) patients, and 10 control subjects. MiRNA expression profiles were determined by quantitative real-time polymerase chain reaction and were analyzed by unsupervised hierarchical cluster analysis. Results: Mean tumour size (±95% confidence interval) in the early and advanced NSCLC patients was 3.3 cm ± 0.9 cm and 4.8 cm ± 0.7 cm respectively. Adenocarcinoma constituted 61.9% of the early and 45.5% of the advanced NSCLC cases respectively. In comparing the early NSCLC group with the control group, the miRNA panel yielded a diagnostic sensitivity of 67% and a specificity of 90.0%. For the advanced NSCLC group, the miRNA panel detected NSCLC with a sensitivity and specificity of 64% and 100% respectively. Conclusions: A sputum MIR-21, MIR-210, and MIR-372 expression profile might provide a sensitive and highly specific means for detecting NSCLC. Sputum miRNA analysis demonstrates promise as a potential complementary screening tool.
Keywords: non-small-cell lung cancer; nsclc; screening; mirna; sputum; rt-pcr non-small-cell lung cancer; nsclc; screening; mirna; sputum; rt-pcr

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MDPI and ACS Style

Razzak, R.; Bédard, E.L.R.; Kim, J.O.; Gazala, S.; Guo, L.; Ghosh, S.; Joy, A.; Nijjar, T.; Wong, E.; Roa, W.H. MicroRNA Expression Profiling of Sputum for the Detection of Early and Locally Advanced Non-Small-Cell Lung Cancer: A Prospective Case–Control Study. Curr. Oncol. 2016, 23, 86-94. https://doi.org/10.3747/co.23.2830

AMA Style

Razzak R, Bédard ELR, Kim JO, Gazala S, Guo L, Ghosh S, Joy A, Nijjar T, Wong E, Roa WH. MicroRNA Expression Profiling of Sputum for the Detection of Early and Locally Advanced Non-Small-Cell Lung Cancer: A Prospective Case–Control Study. Current Oncology. 2016; 23(2):86-94. https://doi.org/10.3747/co.23.2830

Chicago/Turabian Style

Razzak, R., E.L.R. Bédard, J.O. Kim, S. Gazala, L. Guo, S. Ghosh, A. Joy, T. Nijjar, E. Wong, and W.H. Roa. 2016. "MicroRNA Expression Profiling of Sputum for the Detection of Early and Locally Advanced Non-Small-Cell Lung Cancer: A Prospective Case–Control Study" Current Oncology 23, no. 2: 86-94. https://doi.org/10.3747/co.23.2830

APA Style

Razzak, R., Bédard, E. L. R., Kim, J. O., Gazala, S., Guo, L., Ghosh, S., Joy, A., Nijjar, T., Wong, E., & Roa, W. H. (2016). MicroRNA Expression Profiling of Sputum for the Detection of Early and Locally Advanced Non-Small-Cell Lung Cancer: A Prospective Case–Control Study. Current Oncology, 23(2), 86-94. https://doi.org/10.3747/co.23.2830

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