Differences of Gut Microbiota in the Freshwater Blackworm (Lumbriculus variegatus: Oligochaeta) in Two Different Habitat Conditions
Abstract
:1. Introduction
2. Materials and Methods
2.1. Field Sampling
2.2. DNA Extraction
2.3. Pyrosequencing of Bacterial 16S rRNA Genes
2.4. Data Analysis
2.5. Statistical Analysis
3. Results
3.1. Gut Microbiome Diversity
3.2. Dominant Taxa Specificity
3.3. Functional Significance of Core Microflora
4. Discussion
4.1. Gut Bacterial Community
4.2. Differences in Gut Microbiome in Distinct Habitat Conditions
4.3. Limitation and Further Studies
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Environmental Variable | Sampling Site | |
---|---|---|
GP7 | GP8 | |
Location (latitude, longitude) | 37°52′27″ N, 127°31′34″ E | 37°50′03″ N, 127°30′57″ E |
Water velocity (m/s) | 0.3 ± 0.1 | 0.2 ± 0.0 |
Water depth (cm) | 46.9 ± 9.3 | 48.2 ± 10.9 |
Water width (m) | 47.8 ± 2.5 | 77.5 ± 19.3 |
Turbidity (NTU) | 1.3 ± 0.4 | 1.6 ± 0.3 |
Electric conductivity (μS/cm) | 69.8 ± 3.2 | 79.7 ± 4.5 |
pH | 7.5 ± 0.1 | 7.7 ± 0.2 |
Dissolved oxygen (mg/L) | 12.6 ± 0.5 | 12.4 ± 0.5 |
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Kim, P.S.; Lee, Y.-R.; Kwon, Y.-S.; Bae, J.-W.; Lee, S.-J.; Park, Y.-S. Differences of Gut Microbiota in the Freshwater Blackworm (Lumbriculus variegatus: Oligochaeta) in Two Different Habitat Conditions. Int. J. Environ. Res. Public Health 2021, 18, 10298. https://doi.org/10.3390/ijerph181910298
Kim PS, Lee Y-R, Kwon Y-S, Bae J-W, Lee S-J, Park Y-S. Differences of Gut Microbiota in the Freshwater Blackworm (Lumbriculus variegatus: Oligochaeta) in Two Different Habitat Conditions. International Journal of Environmental Research and Public Health. 2021; 18(19):10298. https://doi.org/10.3390/ijerph181910298
Chicago/Turabian StyleKim, Pil Soo, Yeo-Rang Lee, Yong-Su Kwon, Jin-Woo Bae, Sung-Jae Lee, and Young-Seuk Park. 2021. "Differences of Gut Microbiota in the Freshwater Blackworm (Lumbriculus variegatus: Oligochaeta) in Two Different Habitat Conditions" International Journal of Environmental Research and Public Health 18, no. 19: 10298. https://doi.org/10.3390/ijerph181910298