The Dose of Fungal Aerosol Inhaled by Workers in a Waste-Sorting Plant in Poland: A Case Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sampling Site
2.2. Sampling and Analysis Methods
2.3. Identification of Selected Fungi
- (1)
- Growing a pure fungus culture on a Petri dish with MEA 2% until enough conidiation is present to prepare a suspension;
- (2)
- Swabbing the conidia on the surface of the agar plate and suspending to a specified density in FF inoculating fluid;
- (3)
- Adding 100 μl of suspension into each well of the FF MicroPlate;
- (4)
- Incubation of the FF MicroPlate at 26 °C for 24–96 hours;
- (5)
- Reading the MicroPlates using the Biolog MicroStation™.
2.4. Calculation of the Fungal Waste-Sorting Plant Exposure Dose (FWSPED)
3. Results and Discussion
3.1. Fungal Waste-Sorting Plant Exposure Dose (FWSPED)
3.2. Identification of Fungal Aerosol
3.3. Particle Size Distribution (PSD) of Isolated Fungi
4. Conclusions
Funding
Acknowledgments
Conflicts of Interest
References
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PSP | QSP | |
---|---|---|
Ventilation system | Undergoes a 20-fold air exchange every hour (h); the cabins have supply and exhaust ventilation, and the air is drawn away from the conveyor belts. | |
Volume (m3) | 178 | 565 |
Number of occupants | 6 | 10 |
Indoor temperature (°C) | 18.3–21.4 | 17.1–19.4 |
Indoor relative humidity (%) | 18–22 | 26–34 |
Outdoor temperature (°C) Outdoor relative humidity (%) | 10.2–12.2 25.2–28 |
Waste-Sorting Plant (WSP) | ||
---|---|---|
Parameter | Short-Term Inhalation Rates by Activity Level/Minutes | |
Activity Levels | Staff | Staff |
(m3/min) | (hour) | |
Sedentary/passive | 0.0048 | 2 |
Light intensity | 0.013 | 2 |
Moderate intensity | 0.028 | 2 |
High intensity | 0.052 | 2 |
Body weight (kg) | PSP 70; QSP 60 |
FWSPED—Waste-Sorting-Plant EX Dose (CFU/kg) | AVC—Average Concentration (CFU/m3) | |
---|---|---|
PSP | 193 | 8.1 ± 4.0 x 102 |
QSP | 185 | 7.2 ± 3.1 x 102 |
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Brągoszewska, E. The Dose of Fungal Aerosol Inhaled by Workers in a Waste-Sorting Plant in Poland: A Case Study. Int. J. Environ. Res. Public Health 2020, 17, 177. https://doi.org/10.3390/ijerph17010177
Brągoszewska E. The Dose of Fungal Aerosol Inhaled by Workers in a Waste-Sorting Plant in Poland: A Case Study. International Journal of Environmental Research and Public Health. 2020; 17(1):177. https://doi.org/10.3390/ijerph17010177
Chicago/Turabian StyleBrągoszewska, Ewa. 2020. "The Dose of Fungal Aerosol Inhaled by Workers in a Waste-Sorting Plant in Poland: A Case Study" International Journal of Environmental Research and Public Health 17, no. 1: 177. https://doi.org/10.3390/ijerph17010177