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Volume 16
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10.3390/ijerph16122166
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Erratum published on 14 January 2021, see Int. J. Environ. Res. Public Health 2021, 18(2), 678.
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Article

Isolation, Screening, and Characterization of Antibiotic-Degrading Bacteria for Penicillin V Potassium (PVK) from Soil on a Pig Farm

by
Xuanjiang Yang
1,2,
Miao Li
1,*,
Panpan Guo
1,
Hualong Li
1,
Zelin Hu
1,
Xianwang Liu
1 and
Qiang Zhang
3
1
Institute of Intelligent Machinery, Hefei Institute of Material Sciences, Chinese Academy of Sciences, Hefei 230031, Anhui, China
2
Hefei Institutes of Physical Science, Chinese Academy of Sciences, University of Science and Technology of China, Hefei 230026, China
3
Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 5V6, Canada
*
Author to whom correspondence should be addressed.
Int. J. Environ. Res. Public Health 2019, 16(12), 2166; https://doi.org/10.3390/ijerph16122166
Submission received: 26 April 2019 / Revised: 8 June 2019 / Accepted: 10 June 2019 / Published: 19 June 2019
(This article belongs to the Special Issue Microorganisms in the Environment)
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Abstract

(1) Background: Antibiotics are frequently used on farm animals, making animal husbandry a relatively large source of antibiotic pollution of the environment. The present study aims to isolate and acclimatize antibiotic-degrading bacterial strains for penicillin V potassium (PVK) from the contaminated soil of a pig farm. (2) Methods: Bacterial strains were isolated and acclimatized by continuous enrichment of cultures with PVK as the sole carbon source. The antibiotic susceptibility test, thiol mercury salt ultraviolet spectrophotometry (TMSUS), morphological observations, and 16S rDNA sequence analysis were used to identify and characterize the isolated strains. (3) Results: Four bacterial isolates (denoted as LM-1, LM-2, LM-3, LM-4) were obtained, and two of them (LM-1, LM-2) with the highest degradation rates were identified to belong to the same genera as Bacillus. These two isolates were found to be resistant to PVK antibiotic in an antibiotic sensitivity test. The TMSUS indicated that the strains LM-1 and LM-2 had good performance in PVK degradation (68% for LM-1, 66% for LM-2 in 48 h) when the initial PVK concentration was about 100 μg/mL. (4) Conclusions: Two bacterial strains isolated from the soil on a pig farm are effective in degrading PVK and can be potentially used for bioremediation of PVK antibiotic-contaminated soils.
Keywords: penicillin; biodegradation; soil; pig farm; bacterial isolate; Bacillus penicillin; biodegradation; soil; pig farm; bacterial isolate; Bacillus

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MDPI and ACS Style

Yang, X.; Li, M.; Guo, P.; Li, H.; Hu, Z.; Liu, X.; Zhang, Q. Isolation, Screening, and Characterization of Antibiotic-Degrading Bacteria for Penicillin V Potassium (PVK) from Soil on a Pig Farm. Int. J. Environ. Res. Public Health 2019, 16, 2166. https://doi.org/10.3390/ijerph16122166

AMA Style

Yang X, Li M, Guo P, Li H, Hu Z, Liu X, Zhang Q. Isolation, Screening, and Characterization of Antibiotic-Degrading Bacteria for Penicillin V Potassium (PVK) from Soil on a Pig Farm. International Journal of Environmental Research and Public Health. 2019; 16(12):2166. https://doi.org/10.3390/ijerph16122166

Chicago/Turabian Style

Yang, Xuanjiang, Miao Li, Panpan Guo, Hualong Li, Zelin Hu, Xianwang Liu, and Qiang Zhang. 2019. "Isolation, Screening, and Characterization of Antibiotic-Degrading Bacteria for Penicillin V Potassium (PVK) from Soil on a Pig Farm" International Journal of Environmental Research and Public Health 16, no. 12: 2166. https://doi.org/10.3390/ijerph16122166

APA Style

Yang, X., Li, M., Guo, P., Li, H., Hu, Z., Liu, X., & Zhang, Q. (2019). Isolation, Screening, and Characterization of Antibiotic-Degrading Bacteria for Penicillin V Potassium (PVK) from Soil on a Pig Farm. International Journal of Environmental Research and Public Health, 16(12), 2166. https://doi.org/10.3390/ijerph16122166

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