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Marine Drugs
Volume 21
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10.3390/md21030159
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Article

Extraction and Characterization of Pepsin- and Acid-Soluble Collagen from the Swim Bladders of Megalonibea fusca

by
Chou Mo
1,†,
Qiaoli Wang
2,3,4,†,
Guangfeng Li
2,
Wanwen Dong
1,
Feng Liang
3,
Chaoxi Wu
2,5,*,
Zhiping Wang
1,* and
Yifei Wang
1,2,4,5,*
1
Guangdong Provincial Key Laboratory of Advanced Drug Delivery, Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System, Guangdong Pharmaceutical University, Guangzhou 510006, China
2
Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China
3
Guangzhou Jinan Biomedical Research and Development Center Co., Ltd., Guangzhou 510000, China
4
Key Laboratory of Innovative Technology Research on Natural Products and Cosmetics Raw Materials, Guangzhou 510632, China
5
Guangdong Provincial Biotechnology Drug & Engineering Technology Research Center, Guangzhou 510632, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Mar. Drugs 2023, 21(3), 159; https://doi.org/10.3390/md21030159
Submission received: 25 January 2023 / Revised: 23 February 2023 / Accepted: 23 February 2023 / Published: 27 February 2023
(This article belongs to the Special Issue Fundamentals and Biomedical Applications of Marine Collagen)
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Abstract

There is a growing demand for the identification of alternative sources of collagen not derived from land-dwelling animals. The present study explored the use of pepsin- and acid-based extraction protocols to isolate collagen from the swim bladders of Megalonibea fusca. After extraction, these acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) samples respectively were subjected to spectral analyses and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) characterization, revealing both to be comprised of type I collagen with a triple-helical structure. The imino acid content of these ASC and PSC samples was 195 and 199 residues per 1000 residues, respectively. Scanning electron microscopy demonstrated that samples of freeze-dried collagen exhibited a compact lamellar structure, while transmission electron microscopy and atomic force microscopy confirmed the ability of these collagens to undergo self-assembly into fibers. ASC samples exhibited a larger fiber diameter than the PSC samples. The solubility of both ASC and PSC was highest under acidic pH conditions. Neither ASC nor PSC caused any cytotoxicity when tested in vitro, which met one of the requirements for the biological evaluation of medical devices. Thus, collagen isolated from the swim bladders of Megalonibea fusca holds great promise as a potential alternative to mammalian collagen.
Keywords: Megalonibea fusca swim bladders; collagen; characterization; structure Megalonibea fusca swim bladders; collagen; characterization; structure

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MDPI and ACS Style

Mo, C.; Wang, Q.; Li, G.; Dong, W.; Liang, F.; Wu, C.; Wang, Z.; Wang, Y. Extraction and Characterization of Pepsin- and Acid-Soluble Collagen from the Swim Bladders of Megalonibea fusca. Mar. Drugs 2023, 21, 159. https://doi.org/10.3390/md21030159

AMA Style

Mo C, Wang Q, Li G, Dong W, Liang F, Wu C, Wang Z, Wang Y. Extraction and Characterization of Pepsin- and Acid-Soluble Collagen from the Swim Bladders of Megalonibea fusca. Marine Drugs. 2023; 21(3):159. https://doi.org/10.3390/md21030159

Chicago/Turabian Style

Mo, Chou, Qiaoli Wang, Guangfeng Li, Wanwen Dong, Feng Liang, Chaoxi Wu, Zhiping Wang, and Yifei Wang. 2023. "Extraction and Characterization of Pepsin- and Acid-Soluble Collagen from the Swim Bladders of Megalonibea fusca" Marine Drugs 21, no. 3: 159. https://doi.org/10.3390/md21030159

APA Style

Mo, C., Wang, Q., Li, G., Dong, W., Liang, F., Wu, C., Wang, Z., & Wang, Y. (2023). Extraction and Characterization of Pepsin- and Acid-Soluble Collagen from the Swim Bladders of Megalonibea fusca. Marine Drugs, 21(3), 159. https://doi.org/10.3390/md21030159

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