Low-Molecular-Weight Fish Collagen Peptide (Valine-Glycine-Proline-Hydroxyproline-Glycine-Proline-Alanine-Glycine) Prevents Osteoarthritis Symptoms in Chondrocytes and Monoiodoacetate-Injected Rats
Abstract
:1. Introduction
2. Results
2.1. LMWCP Ameliorated Cell Damage and the Expression of Catabolic Factors in H2O2-Treated Chondrocytes
2.2. LMWCP Ameliorated Inflammation in LPS-Treated Chondrocytes
2.3. LMWCP Ameliorated Apoptosis in LPS-Treated Chondrocytes
2.4. LMWCP Ameliorated Morphological Change of Cartilage Tissue in MIA-Injected Rats
2.5. LMWCP Ameliorated Catabolic Factors Expression in Cartilage Tissue from MIA-Injected Rats
2.6. LMWCP Ameliorated Inflammation in Cartilage Tissue from MIA-Injected Rats
2.7. LMWCP Ameliorated Apoptosis in Cartilage Tissue from MIA-Injected Rats
3. Discussion
4. Materials and Methods
4.1. Preparation of LMWCP
4.2. Primary Culture of Chondrocytes
4.3. Animals and Induction of Osteoarthritis
4.4. Micro-CT and ELISA Analysis
4.5. Total RNA Extraction and Real-Time PCR
4.6. Western Blot
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Group | Diet | Supplement | MIA |
---|---|---|---|
Normal control | AIN93G | − | − |
MIA | AIN93G | − | + |
MIA + PC | AIN93G | Ibuprofen 20 mg/kg BW | + |
MIA + 200 | AIN93G | LMWCP 200 mg/kg BW | + |
MIA + 400 | AIN93G | LMWCP 400 mg/kg BW | + |
MIA + 600 | AIN93G | LMWCP 600 mg/kg BW | + |
Gene | Forward Sequence (5′-3′) | Reverse Sequence (5′-3′) |
---|---|---|
GAPDH (NM_017008) | TGG CCT CCA AGG AGT AAG AAA C | CAG CAA CTG AGG GCC TCT CT |
Aggrecan (NM_022190) | GAA GTG GCG TCC AAA CCA A | CGT TCC ATT CAC CCC TCT CA |
Collagen Type I (NM_000088) | GAG CGG AGA GTA CTG GAT CGA | CTG ACC TGT CTC CAT GTT GCA |
Collagen Type II (RATCOLLII) | GCA ACA GCA GGT TCA CGT ACA | TCG GTA CTC GAT GAT GGT CTT G |
TIMP-1 (NM_053819) | ACA GCT TTC TGC AAC TCG GA | CGT CGA ATC CTT TGA GCA TC |
TIMP-3 (RNU27201) | CTT CTG CAA CTC CGA CAT CGT | GGG GCA TCT TAC TGA ATC CTC |
MMP-3 (NM_133523) | GAG TGT GGA TTC TGC CAT TGA G | TTA TGT CAG CCT CTC CTT CAG AGA |
MMP-13 (NM_133530.1) | ACG TTC AAG GAA TCC AGT CT | GGA TAG GGC TGG GTC ACA CTT |
Antibodies | Distributor |
---|---|
Smad3 | Cell signaling (#9523, Beverly, MA, USA) |
p-Smad3 | Cell signaling (#9520) |
MMP-3 | Abcam (ab53015) |
MMP-13 | Abcam (ab39012) |
IκBα | Cell signaling (#9242) |
p-IκBα | Cell signaling (#2859) |
p65 | Abcam (ab16502) |
p-p65 | Cell signaling (#3031) |
COX-2 | Cell signaling (#12282) |
JNK | Cell signaling (#9252) |
p-JNK | Cell signaling (#9251) |
c-Fos | Cell signaling (#4384 |
p-c-Fos | Cell signaling (#5348) |
c-Jun | Cell signaling (#9165) |
p-c-Jun | Cell signaling (#2361) |
Bax | Cell signaling (#2772) |
FADD | LSbio (LS-C766496, Settle, WA, USA) |
Caspase-8 | Cell signaling (#4790) |
Cleaved caspase-8 | Cell signaling (#8592) |
Caspase-3 | Cell signaling (#9662) |
Cleaved caspase-3 | Cell signaling (#9661) |
β-actin | BETHYL (A300-491A, Waltham, MA, USA) |
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Cho, W.; Park, J.; Kim, J.; Lee, M.; Park, S.J.; Kim, K.S.; Jun, W.; Kim, O.-K.; Lee, J. Low-Molecular-Weight Fish Collagen Peptide (Valine-Glycine-Proline-Hydroxyproline-Glycine-Proline-Alanine-Glycine) Prevents Osteoarthritis Symptoms in Chondrocytes and Monoiodoacetate-Injected Rats. Mar. Drugs 2023, 21, 608. https://doi.org/10.3390/md21120608
Cho W, Park J, Kim J, Lee M, Park SJ, Kim KS, Jun W, Kim O-K, Lee J. Low-Molecular-Weight Fish Collagen Peptide (Valine-Glycine-Proline-Hydroxyproline-Glycine-Proline-Alanine-Glycine) Prevents Osteoarthritis Symptoms in Chondrocytes and Monoiodoacetate-Injected Rats. Marine Drugs. 2023; 21(12):608. https://doi.org/10.3390/md21120608
Chicago/Turabian StyleCho, Wonhee, Jeongjin Park, Jinhee Kim, Minhee Lee, So Jung Park, Kyung Seok Kim, Woojin Jun, Ok-Kyung Kim, and Jeongmin Lee. 2023. "Low-Molecular-Weight Fish Collagen Peptide (Valine-Glycine-Proline-Hydroxyproline-Glycine-Proline-Alanine-Glycine) Prevents Osteoarthritis Symptoms in Chondrocytes and Monoiodoacetate-Injected Rats" Marine Drugs 21, no. 12: 608. https://doi.org/10.3390/md21120608