Limited Metabolomic Overlap between Commensal Bacteria and Marine Sponge Holobionts Revealed by Large Scale Culturing and Mass Spectrometry-Based Metabolomics: An Undergraduate Laboratory Pedagogical Effort at Georgia Tech
Abstract
:1. Introduction
2. Results and Discussion
2.1. Marine Sponge Specimens
2.2. Metabolomes of Sponges and Bacterial Isolates
2.3. Phylogeny of Bacterial Isolates
2.4. Dependence of Isolation Media and Source Sponge on Bacterial Metabolomes
2.5. Cryptic Metabolites Are Shared between Sponges and Commensal Bacteria
2.6. Commensal Bacteria Are Sources of Novel Natural Products
2.7. Pedagogic Deliverables
3. Materials and Methods
3.1. Collection of Sponge Specimens
3.2. Bacterial Culturing
3.3. Preparation of Extracts for LC/MS
3.4. LC/MS Data Collection, Preprocessing, and Statistical Analysis
3.5. Genomic DNA Isolation
S rRNA Gene Amplicon Sequencing
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Deutsch, J.M.; Green, M.O.; Akavaram, P.; Davis, A.C.; Diskalkar, S.S.; Du Plessis, I.A.; Fallon, H.A.; Grason, E.M.; Kauf, E.G.; Kim, Z.M.; et al. Limited Metabolomic Overlap between Commensal Bacteria and Marine Sponge Holobionts Revealed by Large Scale Culturing and Mass Spectrometry-Based Metabolomics: An Undergraduate Laboratory Pedagogical Effort at Georgia Tech. Mar. Drugs 2023, 21, 53. https://doi.org/10.3390/md21010053
Deutsch JM, Green MO, Akavaram P, Davis AC, Diskalkar SS, Du Plessis IA, Fallon HA, Grason EM, Kauf EG, Kim ZM, et al. Limited Metabolomic Overlap between Commensal Bacteria and Marine Sponge Holobionts Revealed by Large Scale Culturing and Mass Spectrometry-Based Metabolomics: An Undergraduate Laboratory Pedagogical Effort at Georgia Tech. Marine Drugs. 2023; 21(1):53. https://doi.org/10.3390/md21010053
Chicago/Turabian StyleDeutsch, Jessica M., Madison O. Green, Priyanka Akavaram, Ashleigh C. Davis, Sarth S. Diskalkar, Isabelle A. Du Plessis, Hannah A. Fallon, Emma M. Grason, Emma G. Kauf, Zoe M. Kim, and et al. 2023. "Limited Metabolomic Overlap between Commensal Bacteria and Marine Sponge Holobionts Revealed by Large Scale Culturing and Mass Spectrometry-Based Metabolomics: An Undergraduate Laboratory Pedagogical Effort at Georgia Tech" Marine Drugs 21, no. 1: 53. https://doi.org/10.3390/md21010053