Interactions of Nereistoxin and Its Analogs with Vertebrate Nicotinic Acetylcholine Receptors and Molluscan ACh Binding Proteins
Abstract
:1. Introduction
2. Results
2.1. Effects of NTX on α4β2 Neuronal Receptors
2.2. Effects on α7Neuronal Receptors
2.3. Torpedo Electric Organ nAChR Binding
2.4. Binding of NTX and MeNTX to Molluscan ACh Binding Proteins
3. Discussion
4. Materials and Methods
4.1. NTX Compounds
4.2. Radioligand Binding Assays
4.3. Radioligand Binding Assay for AChBPs
4.4. Nicotinic Receptor FlexStation Functional Assays
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Rat Brain | Rat Brain | Torpedo | |
---|---|---|---|
Compound | α4β2 1 | α7 2 | Electric Organ 2 |
IC50 ± SEM (µM) | |||
NTX | 60 ± 22 (6) | 390 ± 8.6 (4) | 230 ± 71 (3) |
MeNTX | 120 ± 13 (3) | 45 ± 2.3 (2) | 9.3 ± 4.9 (4) |
DMA-DT | 160 ± 96 (3) | 640 ± 220 (2) | >500 (1) |
TMA-DT | 290 ± 60 (4) | 90 ± 4.3 (2) | 150 (1) |
Compound | Ls BP | Ac BP | Ac BP Y55W |
---|---|---|---|
IC50 ± SEM (µM) | |||
NTX | 140 ± 24 (2) | 71 ±1.9 (3) | 110 ± 25 (3) |
MeNTX | 163 ± 8.7 (3) | 260 ± 71 (3) | 74 ± 5.2 (3) |
DMA-DT | 22 ± 2.4 (3) | 28 ± 0.27 (3) | 41.4 ± 4.4 (3) |
TMA-DT | 3.7 ± 0.39 (3) | 20 ± 2.0 (3) | 18.0 ± 2.8 (3) |
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Kem, W.R.; Andrud, K.; Bruno, G.; Xing, H.; Soti, F.; Talley, T.T.; Taylor, P. Interactions of Nereistoxin and Its Analogs with Vertebrate Nicotinic Acetylcholine Receptors and Molluscan ACh Binding Proteins. Mar. Drugs 2022, 20, 49. https://doi.org/10.3390/md20010049
Kem WR, Andrud K, Bruno G, Xing H, Soti F, Talley TT, Taylor P. Interactions of Nereistoxin and Its Analogs with Vertebrate Nicotinic Acetylcholine Receptors and Molluscan ACh Binding Proteins. Marine Drugs. 2022; 20(1):49. https://doi.org/10.3390/md20010049
Chicago/Turabian StyleKem, William R., Kristin Andrud, Galen Bruno, Hong Xing, Ferenc Soti, Todd T. Talley, and Palmer Taylor. 2022. "Interactions of Nereistoxin and Its Analogs with Vertebrate Nicotinic Acetylcholine Receptors and Molluscan ACh Binding Proteins" Marine Drugs 20, no. 1: 49. https://doi.org/10.3390/md20010049