A Novel Gelatinase from Marine Flocculibacter collagenilyticus SM1988: Characterization and Potential Application in Collagen Oligopeptide-Rich Hydrolysate Preparation
Abstract
:1. Introduction
2. Results and Discussion
2.1. Aa2_1884 Is a Novel Multimodular Peptidase of the S8 Family
2.2. Aa2_1884 Is a Gelatinase with High Activity toward Denatured Collagens
2.3. Aa2_1884 Shows High Hydrolytic Efficiency on Bovine Bone Collagen
2.4. The Collagen Hydrolysate Prepared with Aa2_1884 Is Rich in Collagen Oligopeptides
2.5. Antioxidant Activity of Bovine Bone Collagen Hydrolysate
3. Materials and Methods
3.1. Materials
3.2. Sequence Analysis
3.3. Protein Expression and Purification
3.4. Enzyme Assay
3.5. Enzyme Characterization
3.6. Optimization of the Enzymatic Hydrolysis Parameters
3.7. Preparation and Evaluation of Collagen Hydrolysate
3.8. Analysis of the Antioxidant Activity of the Collagen Hydrolysate
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Substrate | Enzymatic Activity (U/mL) |
---|---|
Bovine bone collagen | 801.15 ± 46.45 |
Bovine tendon collagen | 761.27 ± 43.41 |
Gelatin | 834.00 ± 61.39 |
Casein | 51.75 ± 1.85 |
Elastin-orcein | – |
Metal Ion | Relative Activity (%) | Metal Ion | Relative Activity (%) | ||
---|---|---|---|---|---|
2 mM | 4 mM | 2 mM | 4 mM | ||
Control | 100 | 100 | K+ | 108.62 ± 8.24 | 116.60 ± 4.18 |
Ca2+ | 263.67 ± 7.06 | 251.90 ± 10.14 | Sn2+ | 97.01 ± 0.12 | 100.32 ± 4.40 |
Ba2+ | 250.54 ± 11.34 | 276.80 ± 10.14 | Zn2+ | 56.50 ± 2.99 | 40.69 ± 1.33 |
Sr2+ | 245.91 ± 6.45 | 210.87 ± 29.18 | Fe2+ | 43.73 ± 4.78 | 5.05 ± 2.04 |
Mg2+ | 217.58 ± 7.25 | 217.25 ± 32.74 | Ni2+ | – b | – |
Li+ | 111.41 ± 4.40 | 116.60 ± 4.18 | Co2+ | – | – |
Cu2+ | 100.32 ± 2.96 | 122.83 ± 2.43 | Mn2+ | – | – |
Amino Acid | Total Amino Acids (g/100 g) | Free Amino Acids (g/100 g) |
---|---|---|
Ala | 6.87 ± 0.05 | 0.07 ± 0.02 |
Arg | 7.16 ± 0.06 | |
Asp | 3.39 ± 0.03 | 0.10 ± 0.02 |
Cit | 0.07 ± 0.03 | |
Cys | 0.56 ± 0.04 | 0.12 ± 0.01 |
Glu | 4.04 ± 0.03 | |
Gly | 17.21 ± 0.12 | 0.02 ± 0.01 |
His | 0.67 ± 0.02 | |
Ile | 1.54 ± 0.04 | 0.07 ± 0.01 |
Leu | 2.73 ± 0.06 | 0.04 ± 0.01 |
Lys | 3.23 ± 0.05 | |
Met | 0.75 ± 0.06 | 0.10 ± 0.01 |
Orn | 0.17 ± 0.01 | |
Phe | 1.83 ± 0.11 | |
Pro | 10.10 ± 0.08 | |
Ser | 3.03 ± 0.03 | 0.15 ± 0.04 |
Thr | 2.47 ± 0.03 | |
Trp b | – | |
Tyr | 0.79 ± 0.10 | 0.09 ± 0.07 |
Val | 2.10 ± 0.02 | 0.14 ± 0.01 |
Hylys | 1.04 ± 0.05 | 0.19 ± 0.05 |
Hypro | 8.18 ± 0.11 |
Molecular-Weight Range (Da) | Hydrolysate (%) |
---|---|
>10,000 | 9.68 ± 0.13 |
5000–10,000 | 6.25 ± 0.02 |
3000–5000 | 12.46 ± 0.11 |
1000–3000 | 16.49 ± 0.09 |
500–1000 | 15.60 ± 0.07 |
<500 | 39.50 ± 0.20 |
Antioxidant Activity | Hydrolysate Concentration (mg/mL) | Enzyme | Method | Collagen Source | Reference |
---|---|---|---|---|---|
32.8% a | 10 | Aa2_1884 (S8) | Enzymolysis | Bovine bone | This study |
40.7% a | 30 | A69 (M4) | Enzymolysis | Bovine bone | [43] |
341.91% a | 10 | MCP-01 (S8) | Enzymolysis | Fish skin | [7] |
50% a | 8.38 | SWH b | Fish bone | [44] | |
50% a | 7.58 | SWH | Fish skin | [44] | |
50% a | 5.81 | Pepsin | Enzymolysis | Fish skin | [45] |
50% a | 1.57 | Pepsin | Enzymolysis and fraction isolation | Fish skin | [46] |
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Li, J.; Cheng, J.-H.; Teng, Z.-J.; Zhang, X.; Chen, X.-L.; Sun, M.-L.; Wang, J.-P.; Zhang, Y.-Z.; Ding, J.-M.; Tian, X.-M.; et al. A Novel Gelatinase from Marine Flocculibacter collagenilyticus SM1988: Characterization and Potential Application in Collagen Oligopeptide-Rich Hydrolysate Preparation. Mar. Drugs 2022, 20, 48. https://doi.org/10.3390/md20010048
Li J, Cheng J-H, Teng Z-J, Zhang X, Chen X-L, Sun M-L, Wang J-P, Zhang Y-Z, Ding J-M, Tian X-M, et al. A Novel Gelatinase from Marine Flocculibacter collagenilyticus SM1988: Characterization and Potential Application in Collagen Oligopeptide-Rich Hydrolysate Preparation. Marine Drugs. 2022; 20(1):48. https://doi.org/10.3390/md20010048
Chicago/Turabian StyleLi, Jian, Jun-Hui Cheng, Zhao-Jie Teng, Xia Zhang, Xiu-Lan Chen, Mei-Ling Sun, Jing-Ping Wang, Yu-Zhong Zhang, Jun-Mei Ding, Xin-Min Tian, and et al. 2022. "A Novel Gelatinase from Marine Flocculibacter collagenilyticus SM1988: Characterization and Potential Application in Collagen Oligopeptide-Rich Hydrolysate Preparation" Marine Drugs 20, no. 1: 48. https://doi.org/10.3390/md20010048