New Diterpenoids and Isocoumarin Derivatives from the Mangrove-Derived Fungus Hypoxylon sp.

Abstract

Two new diterpenoids, hypoxyterpoids A (1) and B (2), and four new isocoumarin derivatives, hypoxymarins A–D (4–7), together, with seven known metabolites (3 and 8–13) were obtained from the crude extract of the mangrove-derived fungus Hypoxylon sp. The structures of the new compounds were elucidated on the basis of 1- and 2-dimensional (1D/2D) nuclear magnetic resonance (NMR) spectroscopic and mass spectrometric analysis. The absolute configurations of compounds 1, 2, 4, 5, and 7 were determined by comparison of experimental and calculated electronic circular dichroism (ECD) spectra, and the absolute configurations of C-4′ in 6 and C-9 in 7 were determined by [Rh₂(OCOCF₃)₄]-induced ECD spectra. Compound 1 showed moderate α-glucosidase inhibitory activities with IC₅₀ values of 741.5 ± 2.83 μM. Compounds 6 and 11 exhibited DPPH scavenging activities with IC₅₀ values of 15.36 ± 0.24 and 3.69 ± 0.07 μM, respectively.

1. Introduction

Bioactive secondary metabolites have played an important role for drug discovery as lead compounds [1,2]. Fungi especially from unique ecological niches are capable of producing a variety of bioactive compounds [3,4,5]. Mangrove-derived endophytic fungi, as plant mutualists that occur in the tropical and subtropical intertidal estuarine zones, have attracted more attention of natural product chemists due to their production of structurally diverse and biologically active secondary metabolites [6,7,8,9,10,11,12]. Hypoxylon species are widespread in terrestrial and marine environments, and chemical investigations of some Hypoxylon spp. have afforded a variety of natural products, such as azaphilones, diterpenes, sporothriolide derivatives, and various aromatic compounds, which exhibited antibacterial, antifungal, and cytotoxic activities [13,14,15,16,17,18].

As part of our ongoing search for new bioactive natural products from mangrove-derived endophytic fungi [11], a strain of fungus Hypoxylon sp. (Hsl2-6) from Bruguiera gymnorrhiza collected in Fangchenggang City, Guangxi Province, People’s Republic of China, was screened out for investigations. The fungus was fermented on rice medium for 30 days and subsequently extracted by EtOAc to afford the organic extract. Fractionations of this extract were performed, leading to the isolation of two new diterpenoids, hypoxyterpoids A (1) and B (2), and four new isocoumarin derivatives, hypoxymarins A–D (4–7), together with seven known metabolites, penicichrysogene B (3) [19] penicimarin A (8) [20], aspergillumarin A (9) [21], aspergillumarin B (10) [21], 5-hydroxysescandelin (11) [22], sescandelin A (12) [23], and sescandelin B (13) [24] (Figure 1). Herein, the isolation, structure elucidation and bioactivities of these compounds are reported.

2. Results and Discussion

The fungus Hypoxylon sp. (Hsl2-6) was cultured in rice medium at room temperature for 30 days. The EtOAc extracts were combined to afford an organic residue (30.0 g) that was fractionated and purified by column chromatography (CC) on silica gel, RP-18, and semipreparative HPLC columns to acquire compounds 1–13.

Hypoxyterpoid A (1) was obtained as a brown oil. It had a molecular formula of C₂₀H₃₀O₅ (six degrees of unsaturation) on the basis of its high-resolution electrospray ionization mass spectrometry (HRESIMS) [M + Na]⁺ at m/z 373.1995 (calcd for C₂₀H₃₀O₅Na 373.1991). The infrared (IR) spectrum of 1 at 1704, 1644 cm⁻¹ showed the presence of carbonyl and double bond groups. Analysis of the ¹H and ¹³C nuclear magnetic resonance (NMR, Figures S1 and S2) and heteronuclear single quantum correlations (HSQC) data (

Table 1. ¹H NMR and ¹³C NMR data (500 and 125 MHz) for 1 (in DMSO-d₆) and 2 (in Acetone-d₆).

Position	1		2
	δC	δH (J in Hz)	δC	δH (J in Hz)
1a	48.0, CH2	0.87, td (12, 3)	35.8, CH2	2.72, d (18.3)
1b		1.96, m		3.11, d (18.3)
2	62.9, CH	3.88, tt (4.2, 12)	172.8, C
3a	46.9, CH2	0.87, td (12, 3)	173.5, C
3b		2.19, m
4	44.2, qC		55.0, C
5	54.5, CH	1.26, m	54.7, CH	3.50, d (11.3)
6a	25.5, CH2	1.70, m	77.7, CH	4.93, m
6b		1.88, m
7a	38.0, CH2	1.84, m	42.4, CH2	2.38, m
7b		2.34, m		3.05, dd (4.5, 11.3)
8	147.7, qC		142.7, C
9	54.6, CH	1.60, m	47.4, CH	3.18, m
10	40.9, C		51.8, C
11a	21.5, CH2	1.44, m	24.1, CH2	1.47, m
11b		1.62, m		1.77, m
12a	38.9, CH2	2.21, m	40.1, CH2	2.10, m
12b		1.94, m		2.34, m
13	158.6, C		160.1, C
14	116.8, CH	5.54, s	116.5, CH	5.65, s
15	167.6, C		167.7, C
16	18.3, CH3	2.07, s	18.7, CH3	2.13, s
17a	106.8, CH2	4.51, s	113.7, CH2	4.91, m
17b		4.88, s		5.21, s
18	28.7, CH3	1.15, s	13.3, CH3	1.25, s
19	178.3, C		175.9, C
20	13.5, CH3	0.54, s	172.6, C
21			52.0, CH3	3.67, s

) revealed the presence of two carbonyl carbons (δ_C 178.3 and 167.6), two sp³ quaternary carbons (δ_C 44.2 and 40.9), four olefinic carbons (two protonated), three sp³ methine (one oxygenated), six sp³ methylene carbons, and three methyl carbons. These data accounted for all ¹H and ¹³C NMR resonances except for three unobserved exchangeable protons and suggested that 1 was a bicyclic compound. Analysis of ¹H-¹H correlation spectroscopy (COSY) spectrum revealed three spin system: C-1/C-2/C-3, C-5/C-6/C-7, and C-9/C-11/C-12. The heteronuclear multiple bond correlations (HMBC) correlations from H₂-1 to C-3 and C-5, H₂-3 to C-5, H-5 to C-4, C-10 and C-18, and from H₃-18 to C-3, C-4, C-5 and C-19 completed the ring A with the methyl carbon C-18 and the carboxylic carbon C-19 attached to C-4 directly. HMBC correlations from H₃-20 to C-1, C-5 and C-10 established the location of the methyl group C-20 at C-10. The exchangeable proton was located at C-2 supported by the chemical shift value for C-2 (δ_C 62.9). While HMBC correlations from H₂-6 to C-8 and C-10, H-9 to C-8, C-10 and C-20, and from H₂-17 to C-7, C-8 and C-9 established the ring B, fused with ring A at C-5 and C-10. Additional HMBC correlations from H₃-16 to C-12, C-13, C-14 and C-15, from H-14 to C-12 and C-15, from H₂-12 to C-13 and C-14, and from H₂-11 to C-13 established the C-13–C-16 subunit, with C-12 attached to C-13 of the olefin C-13/C-14. The hydroxyl groups were located at C-2, C-15, and C-19 by default supported by the chemical shift value for C-2 (δ_C 62.9), C-15 (δ_C 167.6), and C-19 (δ_C 178.3). Thus, The compound 1 and rel-(1R,3S,4aS,5R,8aS)-5-[(3E)-4-carboxy-3-methylbut-3-en-1-yl]decahydro-3-hydroxy-1,4a-dimethyl-6-methylidenenaphthalene-1-carboxylic [25] shared the same planar structure, as depicted in Figure 1.

The relative configuration of 1 was assigned by Rotating Frame Overhauser Effect Spectroscopy (ROESY) experiment (Figure 2) and, by comparison, with the known compound penicichrysogene B (3). The large J value observed for H-3a/H-2 (12.0 Hz) indicated their trans-diaxial orientations. The ROESY correlations of H-5 with H-1a, H-3a, and H-9, and of H-3a with H₃-18 suggested the α-orientated of these protons, whereas those of H-2 with H-1b and H₃-20 indicated these protons were β-orientated. Additionally, the ROESY correlations of H-14 with H₂-12 suggested the E-configuration of the Δ¹³ double bond. The absolute configuration of 1 was assessed by comparison of the experimental and simulated ECD spectra generated by the time-dependent density functional theory (TDDFT) for two enantiomers (2R,4S,5R,9S,10R)-1 (1a) and (2S,4R,5S,9R,10S)-1 (1b). The experimental ECD spectrum of 1 was nearly identical to the calculated ECD spectrum for 1a (Figure 3), clearly indicating the 2R,4S,5R,9S,10R absolute configuration for 1. Thus, the structure of 1 was elucidated, as depicted in Figure 1.

Hypoxyterpoid B (2) was obtained as a brown oil. Its molecular formula C₂₁H₂₆O₁₀ was established by HRESIMS [M + H]⁺ at m/z 439.1605 (calcd for C₂₁H₂₇O₁₀ 439.1604), indicating the nine degrees of unsaturation. Its ¹H and ¹³C NMR data (Table 1) indicated the presence of five carbonyl carbons (δ_C 175.9, 173.5, 172.8, 172.6, and 167.7, respectively), two quaternary carbons, four olefinic carbons with two protonated, three sp³ methine carbons with one oxygenated, four sp³ methylene carbons, and three methyl carbons (one oxygenated). These data suggested that 2 was also a bicyclic compound. Detailed comparison of the ¹H and ¹³C NMR data of 2 (Table 1, Figures S7 and S8) with those of 1 revealed the same fragment of C-11–C-16, which was confirmed by interpretation of the ¹H-¹H COSY and HMBC data (Figure 2, Figures S9 and S11). However, obvious differences in chemical shifts were also observed for some signals for rings A and B. Specifically, the resonances for methylene carbon C-6 (δ_H/C 1.70; 1.88/25.5) in ring B of 1 were replaced by the oxygenated methine carbon C-6 (δ_H/C 4.93/77.7), which was confirmed by the ¹H-¹H COSY correlations H-5/H-6/H-7 and the HMBC correlations from H-5 to C-9 and C-10, from H-6 to C-5 and C-10, from H₂-17 to C-7, C-8 and C-9, and from H-9 to C-8 and C-10. Other HMBC correlations from H₂-1 to C-2, C-5 and C-10, from H-5 to C-1, C-3, C-4 and C-10 and from H₃-18 to C-3, C-4, C-5 and C-19, as well as the bicyclic feature of 2 established a seven-membered ring (ring A) with the methyl carbon C-18 and the carboxylic carbon C-19 attached at C-4. Additionally, HMBC correlations from H₂-1 and H-9 to C-10 and C-20 suggested the carbonyl carbon C-20 was attached to C-10 directly. While HMBC correlation from H₃-21 to C-20 located the methoxyl group at C-10. On the basis of these data, the gross structure of 2 was established as shown.

The ROESY correlations of H-7b with H-5, H-6 and H-9, and of H-6 with H₃-18 revealed the cofacial relationships of these groups (Figure 2). The relative configuration for C-10 was proposed to be the same as 1 and penicichrysogene B (3) from the biosynthetic considerations. The absolute configuration for 2 was also proposed, by a comparison of the experimental and calculated ECD spectra, for the enantiomers (4S,5R,6R,9S,10R)-2 (2a) and (4R,5S,6S,9R,10S)-2 (2b). The result showed that the calculated spectrum of (4S,5R,6R,9S,10R)-2a agreed with the experimental one (Figure 3), indicating the absolute configuration of 2 to be 4S,5R,6R,9S,10R. Compound 2 is a rare type of new diterpenoid derivative with an anhydride moiety. There are few precedents, in the natural products literature, for this type of structures [26,27,28].

Hypoxymarin A (4) was obtained as a yellow oil. Its molecular formula C₁₄H₁₆O₅ was established by HRESIMS analysis (m/z 263.0925 [M − H]⁻), indicating seven degrees of unsaturation. The IR spectrum of 4 at 3306, 1697, 1658, 1598 cm⁻¹ showed the presence of hydroxy, ester carbonyl and aromatic groups. Analysis of its NMR data (

Table 2. ¹H NMR and ¹³C NMR data (500 and 125 MHz) for 4 and 5 in Acetone-d₆.

Position	4		5
	δC	δH (J in Hz)	δC	δH (J in Hz)
1	158.6, C		158.6, C
3	161.6, C		161.3, C
4	103.5, CH	6.22, s	103.8, CH	6.20, s
4a	143.2, C		143.3, C
5	103.6, CH	6.43, s	103.5, CH	6.42, s
6	164.6, C		164.6, C
7	99.4, CH	6.51, s	99.4, CH	6.51, s
8	164.7 C		164.7, C
8a	102.8, C		102.9, C
9	46.4, CH	2.45, m	46.7, CH	2.53, m
10	69.1, CH	3.98, m	69.3, CH	3.98, m
11	13.7, CH3	1.26, d (7.0)	14.2, CH3	1.19, d (7.0)
12	22.0, CH3	1.16, d (6.3)	20.7, CH3	1.17, d (6.2)
13	56.2, CH3	3.87, s	56.3, CH3	3.87, s
OH-6		9.63, br s		9.59, br s

, Figures S13 and S14) and revealed the presence of one exchangeable proton (δ_H 9.63), three methyl groups (including one methoxy), two sp³ methines (one oxygenated), eight aromatic or olefinic carbons (three protonated), and one carbonyl carbon. These data accounted for all ¹H and ¹³C NMR resonances except for one unobserved exchangeable proton. The remaining two degrees of unsaturation suggested that 4 was a bicyclic compound. The ¹H-¹H correlations (Figure 3) of H-9 with H-10 and H₃-11, and of H-10 with H₃-12 allowed the assignment of the C-11–C-9–C-10–C-12 butan-2-ol subunit. The HMBC correlations (Figure 3) from H-4 (δ_H 6.22) to C-3 and C-9, H-9 to C-3 and C-4, and from H-10 and H₃-11 to C-3 indicated that C-9 was directly attached to C-3 of the trisubstituted olefin C-3/C-4. Additional HMBC correlations from H-4 to C-1, C-4a, C-8a and C-5, H-5 to C-4, C-6, C-7, and C-8a, and from H-7 to C-1, C-5, C-6 (δ_C 164.7), C-8 and C-8a, as well as from the methoxy group H₃-13 to C-8 established the isochromenone core structure, with the methoxy group carbon C-13 located at C-8. The remaining two exchangeable protons were located at C-6 and C-10 by default. Thus, the planar structure of 4 was established as shown (Figure 1).

The absolute configuration for 4 was also assigned by ECD calculations. The calculated ECD spectra were obtained by the time-dependent density functional theory (TD-DFT) at the B3LYP/6-311G (2d, p) level for four stereoisomers (9S,10S)-4 (4a), (9R,10R)-4 (4b), (9R,10S)-4 (4c) and (9S,10R)-4 (4d). The overall calculated ECD spectrum of 4a–4d were generated according to Boltzmann weighting of the conformers. The experimental ECD spectrum of 4 was nearly identical to the calculated ECD spectrum for (9S,10S)-4 (4a) (Figure 3), indicating the absolute configuration to be 9S,10S-4.

Hypoxymarin B (5) was obtained as a yellow oil. The molecular formula was determined as C₁₄H₁₆O₅ (seven degrees of unsaturation) by HRESIMS (m/z 263.0924 [M − H]⁻), which is the same with that of 4. The ¹H and ¹³C NMR data (Table 2, Figures S19 and S20) and 2D NMR were very similar, indicating that 5 was a stereoisomer of 4. Subsequent comparison of the experimental ECD spectra of 5, and the calculated ECD spectrum for (9R,10S)-4 (4c), unambiguously assigned its absolute configuration as 9R,10S. Thus, the structure of 5 was elucidated, as depicted in Figure 1.

The molecular formula of hypoxymarin C (6) was determined to be C₁₄H₁₈O₅ (six degrees of unsaturation) by HRESIMS (m/z 267.1226 [M + H]⁺). In the ¹H NMR spectrum (

Table 3. ¹H NMR and ¹³C NMR data (500 and 125 MHz) for 6 (in Acetone-d₆) and 7 (in DMSO-d₆).

Position	6		Position	7
	δC	δH (J in Hz)		δC	δH (J in Hz)
1	170.8, C		1	169.1, C
3	80.5, CH	4.63, m	3	68.3, CH2	4.51, dd (4.1, 11.6) 4.61, dd (1.8, 11.6)
4	27.4, CH2	2.68, dd (3.4, 16.8) 3.19, dd (10.6, 16.8)	4	43.2, CH	2.79, m
4a	125.6, C		4a	143.0, C
5	146.3, C		5	108.2, CH	6.26, d (2.2)
6	124.7, CH	7.12, d (8.8)	6	164.5, C
7	116.1, CH	6.71, d (8.8)	7	101.2, CH	6.19, d (2.2)
8	156.2, C		8	163.1, C
8a	109.3, C		8a	100.1, C
1′	35.7, CH2	1.79, m 1.89, m	9	68.0, CH	3.85, m
2′	22.0, CH2	1.62, m	10	19.9, CH3	1.04, d (6.2)
3′	39.3, CH2	1.48, m	8-OH		11.16, s
4′	67.4, CH	3.77, m
5′	24.1, CH3	1.14, d (6.2)
8-OH		10.6, s

), the proton signals and the coupling constants at δ_H 7.12 (d, J = 8.8 Hz), 6.71 (d, J = 8.8 Hz) indicated the presence of a 1,2,3,6-tetrasubstituted benzene system. Analysis of its NMR data (Table 3, Figures S25 and S26) the presence of one methyl group, four methylenes, two oxygenated methines, six aromatic carbons (two protonated) and one carbonyl carbon (δ_C 170.8). The NMR data of 6 resembled those of aspergillumarin B (10) [21] except that the proton H-5 in 10 were replaced by hydroxyl group in 6, which was confirmed by the chemical shift of C-5 (δ_C 146.3) and the HMBC correlations from H₂-4 and H-6 to C-5. On the basis of these results, the planar structure of 6 was elucidated (Figure 1). In order to determine the absolute configuration of C-4′ at the side chain, a [Rh₂(OCOCF₃)₄]-induced ECD experiment was applied (Figure S34). In the induced ECD spectrum, the positive Cotton effect, at 350 nm, indicated a 4′S configuration according to the bulkiness rule [29]. By comparison with the dihydroisocoumarin data described in the literature [20,21], the negative circular dichroism at 259 nm indicated the R configuration at C-3. Thus, the absolute configuration of 6 was established as 3R,4′S.

Hypoxymarin D (7) was isolated as a yellow oil with the molecular formula assigned as C₁₁H₁₂O₅ on the basis of its HREIMS (m/z 223.0614 [M − H]⁻), indicating six degrees of unsaturation. Analysis of the ¹H and ¹³C NMR data (Table 3, Figures S28 and S29) revealed the presence of one methyl, one oxygenated methylene, two methines (one oxygenated), six aromatic carbons with two protonated, and one carbonyl carbon (δ_C 169.1). The ¹H and ¹³C NMR data of 7 were almost consistent with penicimarin A (8). Further analysis of its ¹H-¹H COSY correlations (Figure 4) of H-9 with H₃-10 and H-4, and of H₂-3 with H-4, as well as the HMBC correlations (Figure 4) from H₃-10 to C-9, from H-9 to C-3, C-4 and C-4a, from H₂-3 to C-1, C-4 and C-4a, from H-4 to C-5 and C-8a, and from H-5 and H-6 to C-7 indicated that 7 and 8 share the same planar structure.

By treating 7 with Rh₂(OCOCF₃)₄ in anhydrous CH₂Cl₂, a negative Cotton effect at 350 nm in the ECD spectrum was observed for the complex (Figure S34). Therefore, successful implementation of the bulkiness rule allowed C-9 to be assigned as R. The absolute configuration of C-4 was further determined by a comparison of the experimental and calculated ECD spectra. The calculated spectrum of (4S,9R)-7a agreed with the experimental one (Figure 3), indicating the absolute configuration of 7 to be 4S,9R.

The known compounds, penicichrysogene B (3) [19] penicimarin A (8) [20], aspergillumarin A (9) [21], aspergillumarin B (10) [21], 5-hydroxysescandelin (11) [22], sescandelin A (12) [23], and sescandelin B (13) [24] (Figure 1) were determined by comparison of their spectroscopic data with those in the literature.

The antibacterial activities of all isolated compounds were evaluated with Bacillus subtilis, Escherichia coli, and Staphylococcus aureus. However, these compounds showed no antibacterial activities against B. subtilis, E. coli, and S. aureus at the concentration of 200 μg/mL. All of the isolated compounds were also evaluated for radical-scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. Compounds 6 and 11 showed free radical scavenging activity with IC₅₀ values of 15.36 ± 0.24 and 3.69 ± 0.07 μM, respectively (ascorbic acid as the positive control with IC₅₀ values of 20.49 ± 0.43 μM). The α-glucosidase inhibitory effects of these compounds were evaluated along with the clinical α-glucosidase inhibitor acarbose. Compound 1 exhibited moderate inhibitory effects against α-glucosidase with IC₅₀ value of 741.50 ± 2.83 μM (acarbose as positive control with IC₅₀ value of 636.80 ± 1.49 μM).

Penicichrysogene B (3) was investigated in the anti-platelets assay and showed no inhibitory activities against AChE and BuChE [19]. Isocoumarin derivatives 8–13 was evaluated for antibacterial activities and cytotoxic activities in vitro, while only compounds 9 and 10 showed weak antibacterial activity against Staphylococcus aureus and Bacillus subtilis [20,21,22,23,24,30]. It is the first time, for 11, that the antioxidant activity has been evaluated in our study.

3. Experimental Section

3.1. General Experimental Procedure

Optical rotations were measured with an Anton Paar MCP 200 Automatic Polarimeter (Anton Paar GmbH, Graz, Austria). Infrared spectra were obtained on a Nicolet IS5 FT-IR spectrophotometer (Thermo Scientific, Madison, WI, USA). The NMR data were measured on a Bruker Avance-500 MHz spectrometer (Bruker, Rheinstetten, Germany). The UV data were recorded on a Thermo Scientific Genesys 10S spectrophotometer (Thermo Scientific, Madison, WI, USA). ECD spectra were acquired on an Applied Photophysics Chirascan spectropolarimeter (Applied Photophysics Ltd., Leatherhead, UK). Mass data were performed on an Agilent Accurate-Mass-Q-TOF LC/MS 6520 instrument (Agilent Technologies, Santa Clara, CA, USA). Preparative HPLC was conducted with an Agilent 1200 HPLC system using a C₁₈ column (Reprosil-Pur Basic C₁₈ column; 5 μm; 10 × 250 mm) with a flow rate of 2.0 mL/min. Preparative HPLC was performed on the Waters system, using a C₁₈ column (SunFire C₁₈ column; 5 μm; 19 × 250 mm), with a flow rate of 15 mL/min. The absorbance of contents, in the 96-well clear plate, was detected by a SpectraMax Paradigm microplate reader (Molecular Devices, Sunnyvale, CA, USA).

3.2. Strain and Fermentation

Hypoxylon sp. (Hsl2-6) was isolated from the branches of Bruguiera gymnorrhiza collected in Beilun River Mouth, Fangchenggang City, Guangxi Province, P. R. China. The isolated strain was identified by sequence analysis (Genbank Accession Number MN547522) of the rDNA internal transcribed spacer (ITS) region. The strain was first placed on potato-dextrose agar medium and cultured at 25 °C for 7 days. Then the agar was cut into grain size to inoculate in four conical flasks (500 mL), each containing 100 mL autoclave sterilized potato dextrose broth. Seed culture medium was cultured at 25 °C and 180 rpm for 7 days. Finally, Erlenmeyer flasks (500 mL) containing 80 g of rice, 120 mL of distilled H₂O, and 10.0 mL seed culture incubated at 25 °C for 30 days.

3.3. Extraction and Isolation

At the end of the fermentation, the rice culture was extracted with EtOAc (three times, each 12 L) and vacuum-dried to afford the crude extract (30.0 g). The extract was fractionated by ODS column chromatography (CC), eluted with 10–100% MeOH to afford twelve fractions (Frs.1–12). The fractions Frs.1–5 (8.7 g) were combined and subjected to silica gel CC and eluted with CHCl₃/MeOH (20:1–5:1) to afford five subfractions (Frs.1.1–1.5). The subfraction Fr.1.1 (1.8 g) was subjected to Sephadex LH-20 CC, eluted with CHCl₃/MeOH (2:1) to generate five fractions (Frs.1.1.1–1.1.5). Fr.1.1.2 (0.7 g) was loaded on normal pressure silica gel CC with petroleum ether (PE)/acetone (200:1–5:1) to yield Frs.1.1.2.1–1.1.2.6. The Fr.1.1.2.5 was further purified by RP HPLC (50% MeOH in H₂O with 0.1% HCOOH; 15.0 mL/min) to afford 9 (9.0 mg, t_R 29.3 min). The subfraction Fr.1.2 (2.0 g) was loaded on Sephadex LH-20 CC, eluted with CHCl₃/MeOH (2:1), to afford five tertiary fractions (Frs.1.2.1–1.2.5). The precipitation 11 (30.0 mg) was obtained from Fr.1.2.2. The remaining of Fr.1.2.2. was then purified by RP HPLC (40% MeOH in H₂O with 0.1% HCOOH; 15.0 mL/min) to afford 13 (2.0 mg, t_R 19.0 min) and 12 (3.0 mg, t_R 20.8 min). Fr.1.2.4 (0.8 g) was fractionated by normal pressure silica gel CC with PE/acetone (15:1–5:1) to afford three fractions (Frs.1.2.4.1–1.2.4.3). Fr.1.1.4.2 was purified by RP HPLC (36% MeOH in H₂O with 0.1% HCOOH; 15.0 mL/min) to afford 7 (5.0 mg, t_R 18.0 min) and 8 (8.1 mg, t_R 21.0 min). The subfraction Fr.1.3 (100.2 mg) was loaded on Sephadex LH-20 CC eluting with CHCl₃/MeOH (2:1) to afford seven fractions (Frs.1.3.1–1.3.7). Fr.1.3.7 was further purified by RP HPLC (42% MeOH in H₂O with 0.1% HCOOH; 2.0 mL/min) to afford 6 (5.7 mg, t_R 24.0 min). Fr.1.5 (1.6 g) was loaded on Sephadex LH-20 CC eluted with CHCl₃/MeOH (2:1) to afford Frs.1.5.1–1.5.3. Fr.1.5.2 was further purified by RP HPLC (45% MeOH in H₂O with 0.1% HCOOH; 15.0 mL/min) to afford 4 (5.0 mg, t_R 15.0 min) and 5 (2.5 mg, t_R 18.0 min). The subfraction Fr.6.2 was purified by RP HPLC (50% MeOH in H₂O with 0.1% HCOOH; 2.0 mL/min) to afford 2 (9.2 mg, t_R 23.0 min) and 10 (4.3 mg, t_R 31.5 min). The fraction Fr.8 was purified by RP HPLC (60% MeOH in H₂O with 0.1% HCOOH; 2.0 mL/min) to afford 1 (3.0 mg, t_R 31.9 min) and 3 (3.0 mg, t_R 45.5 min).

Hypoxyterpoid A (1): brown oil; [α${]}_D^{25}$ +28.8 (c 0.15, MeOH); UV (MeOH) λ_max (log ε): 200 (2.44) nm; IR (neat) ν_max 2941, 2851, 1703, 1644, 1446, 1384, 1231, 1155, 1025, 1003, 889, 824, 762 cm⁻¹; CD (MeOH) λ_max (Δε) 202 (−0.56), 222 (+1.96), 287 (−0.33); ¹H and ¹³C NMR data (500 MHz, DMSO-d₆) see Table 1; HRESIMS: m/z 373.1995 [M + Na]⁺ (calcd for C₂₀H₃₀O₅Na 373.1991).

Hypoxyterpoid B (2): brown oil; [α${]}_D^{25}$ +28.2 (c 0.85, MeOH); UV (MeOH) λ_max (log ε): 200 (3.02); 210 (2.20) nm; IR (neat) ν_max 2952, 1779, 1726, 1644, 1435, 1219, 1165, 1115, 1008, 968, 907 cm⁻¹; CD (MeOH) λ_max (Δε) 200 (−12.61), 215 (+7.96); ¹H and ¹³C NMR data (500 MHz, Acetone-d₆) see Table 1; HRESIMS: m/z 439.1605 [M + H]⁺ (calcd for C₂₁H₂₇O₁₀ 439.1604).

Hypoxymarin A (4): Yellow oil; [α${]}_D^{25}$ −0.6 (c 0.50, MeOH); UV (MeOH) λ_max (log ε): 245 (0.69); 324 (0.13) nm; IR (neat) ν_max 3306, 2975, 1697, 1658, 1598, 1440, 1369, 1237, 1200, 1174, 1123, 1074, 1026, 947, 907, 855 cm⁻¹; CD (MeOH) λ_max (Δε) 200 (+1.47), 209 (−4.52), 231 (+1.79), 278 (+2.42) nm; ¹H and ¹³C NMR data (500 MHz, Acetone-d₆) see Table 2; HRESIMS m/z 263.0925 [M − H]⁻ (calcd for C₁₄H₁₅O₅, 263.0919).

Hypoxymarin B (5): Yellow oil; [α${]}_D^{25}$ + 26.4 (c 0.25, MeOH); UV (MeOH) λ_max (log ε): 245 (1.27); 324 (0.21) nm; IR (neat) ν_max 3420, 2976, 1693, 1656, 1598, 1496, 1441, 1369, 1302, 1238, 1199, 1176, 1121, 1078, 1026, 966, 833, 793cm⁻¹; CD (MeOH) λ_max (Δε) 201 (+1.33), 243 (−3.61), 320 (−1.27) nm; ¹H and ¹³C NMR data (500 MHz, Acetone-d₆) see Table 2; HRESIMS m/z 263.0924 [M − H]^– (calcd for C₁₄H₁₅O₅, 263.0919).

Hypoxymarin C (6): White powder; [α${]}_D^{25}$ –37.2 (c 0.50, MeOH); UV (MeOH) λ_max (log ε): 228 (3.06); 247 (1.73) nm; IR (neat) ν_max 3218, 2962, 2929, 2860, 1657, 1590, 1471, 1406, 1354, 1286, 1127, 1065, 1013, 944, 820, 701 cm⁻¹; CD (MeOH) λ_max (Δε) 200 (−2.01), 221 (+1.96), 260 (−3.18), 283 (+0.75) nm; ¹H and ¹³C NMR data (500 MHz, Acetone-d₆) see Table 3; HRESIMS m/z 267.1226 [M + H]⁺ (calcd for C₁₄H₁₉O₅, 267.1227).

Hypoxymarin D (7): Yellow oil; [α${]}_D^{25}$ −30.8 (c 0.50, MeOH); UV (MeOH) λ_max (log ε): 200 (2.54), 268 (0.36), 302 (0.19) nm; IR (neat) ν_max 3140, 2977, 1662, 1628, 1466, 1400, 1323, 1248, 1167, 1130, 1092, 1047, 1024, 1001, 852, 824, 761 cm⁻¹; CD (MeOH) λ_max (Δε) 209 (−13.17), 233 (+9.00), 248 (+1.59), 268 (+15.77) nm; ¹H and ¹³C NMR data (500 MHz, Acetone-d₆) see Table 3; HRESIMS m/z 223.0614 [M − H]⁻ (calcd for C₁₁H₁₁O₅, 223.0606).

3.4. Antimicrobial Assay

All compounds were tested for antibacterial activities against three pathogenic bacteria (S. aureus CGMCC1.2465, B. subtilis ATCC6663, and E. coli CGMCC1.2340) in 96-well microplates, according to the method of Fan et al. [31]. Ampicillin was used as positive controls, and DMSO was used as a negative control. Repeated all experiments three times.

3.5. DPPH Assay

The scavenging activity of DPPH radical was measured as described in previous literature [31]. The scavenging ability was calculated as follows: scavenging ability (%) = (1-A517 of sample/A517 of control) × 100. Ascorbic acid was used as a positive control, and DMSO was used as a negative control. All experiments were repeated three times.

3.6. α-Glucosidase Inhibitory Assay

First, 10.88 U/mL of α-glucosidase from Saccharomyces cerevisiae was diluted with 0.1 M phosphate buffer to 0.2 U/mL. The assay was performed in a 50 µL reaction system, which contains 10 µL of diluted enzyme solution, 20 µL of 0.1 M phosphate buffer, and 10 µL of dimethyl sulfoxide (DMSO) or sample (dissolved in DMSO). After 10 min of incubation in 96-well plates at 37 °C, a 10 μL portion of 4 mM 4-nitrophenyl-α-d-glucopyranoside (PNPG) was added as a substrate to begin the enzymatic reaction (final concentrations of 50, 100, 200, 400, 800 μM). The plate was incubated for an additional 30 min at 37 °C, and the reaction was quenched by adding 50 μL of 0.2 M Na₂CO₃. The optical density (OD) was measured at an absorbance wavelength of 405 nm using a Spectra Max 190 microplate reader (Molecular Devices, Sunnyvale, CA, USA). Each assay was repeated three times, and acarbose was used as the positive control.

3.7. Rh₂(OCOCF₃)₄-Induced ECD Experiments of 6 and 7

The sample of 6 and 7 (0.5 mg) was dissolved in a dry solution of the stock [Rh₂(OCOCF₃)₄] complex (1.0 mg) in CDCl₃ (200 μL) and was received, to CD measurements, at a concentration of 2.5 mg/mL. The first ECD spectrum was recorded immediately after mixing, and its time evolution was monitored until stationary (30 min after mixing). The inherent CD was subtracted. The absolute configurations of the secondary alcohols in 6 and 7 were determined [29].

3.8. ECD Calculation

Conformational analyses for compound 1, 2, 4, and 7 were performed using Maestro 10.2 in the OPLS3 molecular mechanics force-field within an energy window of 3.0 kcal/mol. All of the conformers were then further optimized by the DFT methods at the B3LYP/6-311G(2d,p) level in the Gaussian 09 software package, respectively [32]. The TD-DFT methods at the B3LYP/6-311G(2d,p) were applied to calculate the 60 lowest electronic transitions, which obtained conformers in vacuum, respectively. The Gaussian function was applied to simulate the ECD spectrum of the conformers. The calculated ECD spectra were obtained according to the Boltzmann weighting of each conformer’s ECD spectrum in MeOH solution.

4. Conclusions

In conclusion, two new diterpenoids, hypoxyterpoids A and B (1 and 2), and four new isocoumarin derivatives, hypoxymarins A–D (4–7), together with seven known metabolites (3 and 8–13), were isolated from the fermentation broth of the mangrove-derived fungus Hypoxylon sp. The structures of the new compounds were elucidated on the basis of NMR spectroscopic and mass spectrometric analysis. The absolute configurations of compounds 1, 2, 4, 5, and 7 were determined by comparison of experimental and calculated ECD spectra, and the absolute configurations of C-4′ in compound 6 and C-9 in compound 7 were determined by [Rh₂(OCOCF₃)₄]-induced ECD spectra. Compound 1 showed moderate α-glucosidase inhibitory activities with IC₅₀ values of 741.5 ± 2.83 μM. Compounds 6 and 11 exhibited potent DPPH scavenging activities with IC₅₀ values of 15.36 ± 0.24 and 3.69 ± 0.07 μM, respectively. Our findings also suggest that the fungal genus Hypoxylon spp. are a rich source of bioactive secondary metabolites, and thus worthy of in-depth investigations.