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Article

Characterization of the CAZy Repertoire from the Marine-Derived Fungus Stemphylium lucomagnoense in Relation to Saline Conditions

1
Biodiversité et Biotechnologie Fongiques, Aix-Marseille Université, INRAE, UMR1163, 13288 Marseille, France
2
Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Ecole Nationale d’Ingénieurs de Sfax, Université de Sfax, Sfax 3029, Tunisia
3
INRAE, Aix-Marseille Université, UMR1163, CIRM-CF, 13288 Marseille, France
4
Institute of Bioinformatics, International Technology Park, Bangalore 560066, India
5
Manipal Academy of Higher Education, Manipal 576104, India
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Architecture et Fonction des Macromolécules Biologiques, Centre National de la Recherche Scientifique, Aix-Marseille Université, 13288 Marseille, France
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USC AFMB, Institut National de Recherche Agronomique, 13288 Marseille, France
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AgroParisTech, Micalis Institute, PAPPSO, Université Paris-Saclay, INRAE, 78350 Jouy-en-Josas, France
9
Institut Jean-Pierre Bourgin, INRAE, AgroParisTech, CNRS, Université Paris-Saclay, 78000 Versailles, France
10
Department of Biological Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia
*
Author to whom correspondence should be addressed.
Mar. Drugs 2020, 18(9), 461; https://doi.org/10.3390/md18090461
Received: 21 July 2020 / Revised: 2 September 2020 / Accepted: 7 September 2020 / Published: 9 September 2020
Even if the ocean represents a large part of Earth’s surface, only a few studies describe marine-derived fungi compared to their terrestrial homologues. In this ecosystem, marine-derived fungi have had to adapt to the salinity and to the plant biomass composition. This articles studies the growth of five marine isolates and the tuning of lignocellulolytic activities under different conditions, including the salinity. A de novo transcriptome sequencing and assembly were used in combination with a proteomic approach to characterize the Carbohydrate Active Enzymes (CAZy) repertoire of one of these strains. Following these approaches, Stemphylium lucomagnoense was selected for its adapted growth on xylan in saline conditions, its high xylanase activity, and its improved laccase activities in seagrass-containing cultures with salt. De novo transcriptome sequencing and assembly indicated the presence of 51 putative lignocellulolytic enzymes. Its secretome composition was studied in detail when the fungus was grown on either a terrestrial or a marine substrate, under saline and non-saline conditions. Proteomic analysis of the four S. lucomagnoense secretomes revealed a minimal suite of extracellular enzymes for plant biomass degradation and highlighted potential enzyme targets to be further studied for their adaptation to salts and for potential biotechnological applications. View Full-Text
Keywords: marine-derived fungus; Stemphylium lucomagnoense; saline adaptation; lignocellulose-degrading enzymes; secretome marine-derived fungus; Stemphylium lucomagnoense; saline adaptation; lignocellulose-degrading enzymes; secretome
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MDPI and ACS Style

Ben Ali, W.; Navarro, D.; Kumar, A.; Drula, E.; Turbé-Doan, A.; Correia, L.O.; Baumberger, S.; Bertrand, E.; Faulds, C.B.; Henrissat, B.; Sciara, G.; Mechichi, T.; Record, E. Characterization of the CAZy Repertoire from the Marine-Derived Fungus Stemphylium lucomagnoense in Relation to Saline Conditions. Mar. Drugs 2020, 18, 461. https://doi.org/10.3390/md18090461

AMA Style

Ben Ali W, Navarro D, Kumar A, Drula E, Turbé-Doan A, Correia LO, Baumberger S, Bertrand E, Faulds CB, Henrissat B, Sciara G, Mechichi T, Record E. Characterization of the CAZy Repertoire from the Marine-Derived Fungus Stemphylium lucomagnoense in Relation to Saline Conditions. Marine Drugs. 2020; 18(9):461. https://doi.org/10.3390/md18090461

Chicago/Turabian Style

Ben Ali, Wissal, David Navarro, Abhishek Kumar, Elodie Drula, Annick Turbé-Doan, Lydie O. Correia, Stéphanie Baumberger, Emmanuel Bertrand, Craig B. Faulds, Bernard Henrissat, Giuliano Sciara, Tahar Mechichi, and Eric Record. 2020. "Characterization of the CAZy Repertoire from the Marine-Derived Fungus Stemphylium lucomagnoense in Relation to Saline Conditions" Marine Drugs 18, no. 9: 461. https://doi.org/10.3390/md18090461

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