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Open AccessArticle

Blue Mussel-Derived Peptides PIISVYWK and FSVVPSPK Trigger Wnt/β-Catenin Signaling-Mediated Osteogenesis in Human Bone Marrow Mesenchymal Stem Cells

by Yunok Oh 1,†, Chang-Bum Ahn 2,† and Jae-Young Je 3,*
1
Institute of Marine Life Sciences, Pukyong National University, Busan 48613, Korea
2
Division of Food and Nutrition, Chonnam National University, Gwangju 61186, Korea
3
Department of Marine-Bio Convergence Science, Pukyong National University, Busan 48547, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Mar. Drugs 2020, 18(10), 510; https://doi.org/10.3390/md18100510
Received: 20 August 2020 / Revised: 24 September 2020 / Accepted: 8 October 2020 / Published: 9 October 2020
(This article belongs to the Special Issue Marine Organisms for Bone Regeneration - 2020)
Marine-derived bioactive peptides have shown potential bone health promoting effects. Although various marine-derived bioactive peptides have potential nutraceutical or pharmaceutical properties, only a few of them are commercially available. This study presented an osteogenic mechanism of blue mussel-derived peptides PIISVYWK and FSVVPSPK as potential bone health promoting agents in human bone marrow-derived mesenchymal stem cells (hBMMSCs). Alkaline phosphatase (ALP) activity and mineralization were stimulated using PIISVYWK and FSVVPSPK as early and late markers of osteogenesis in a concentration-dependent manner. Western blot and RT-qPCR results revealed that PIISVYWK and FSVVPSPK increased osteoblast differentiation of hBMMSCs by activating canonical Wnt/β-catenin signaling-related proteins and mRNAs. Immunofluorescence images confirmed nuclear translocation of β-catenin in osteogenic differentiation. Treatment with the pharmacological inhibitor DKK-1 blocked PIISVYWK- and FSVVPSPK-induced ALP activity and mineralization, as well as mRNA expression of the canonical Wnt/β-catenin signaling pathway in hBMMSC differentiation into osteoblasts. These findings suggested that PIISVYWK and FSVVPSPK promoted the canonical Wnt/β-catenin signaling pathway in osteogenesis of hBMMSCs. Blue mussel-derived PIISVYWK and FSVVPSPK might help develop peptide-based therapeutic agents for bone-related diseases. View Full-Text
Keywords: osteogenic peptides; osteoblast differentiation; Wnt/β-catenin signaling; human mesenchymal stem cells osteogenic peptides; osteoblast differentiation; Wnt/β-catenin signaling; human mesenchymal stem cells
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Oh, Y.; Ahn, C.-B.; Je, J.-Y. Blue Mussel-Derived Peptides PIISVYWK and FSVVPSPK Trigger Wnt/β-Catenin Signaling-Mediated Osteogenesis in Human Bone Marrow Mesenchymal Stem Cells. Mar. Drugs 2020, 18, 510.

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