Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides
AbstractIn this study, three synthetic zinc-chelating peptides (ZCPs) derived from sea cucumber hydrolysates with limited or none of the common metal-chelating amino-acid residues were analyzed by flame atomic absorption spectroscopy, circular dichroism spectroscopy, size exclusion chromatography, zeta-potential, Fourier transform infrared spectroscopy, Raman spectroscopy and nuclear magnetic resonance spectroscopy. The amount of zinc bound to the ZCPs reached maximum values with ZCP:zinc at 1:1, and it was not further increased by additional zinc presence. The secondary structures of ZCPs were slightly altered, whereas no formation of multimers was observed. Furthermore, zinc increased the zeta-potential value by neutralizing the negatively charged residues. Only free carboxyl in C-terminus of ZCPs was identified as the primary binding site of zinc. These results provide the theoretical foundation to understand the mechanism of zinc chelation by peptides. View Full-Text
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Liu, X.; Wang, Z.; Yin, F.; Liu, Y.; Qin, N.; Nakamura, Y.; Shahidi, F.; Yu, C.; Zhou, D.; Zhu, B. Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides. Mar. Drugs 2019, 17, 438.
Liu X, Wang Z, Yin F, Liu Y, Qin N, Nakamura Y, Shahidi F, Yu C, Zhou D, Zhu B. Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides. Marine Drugs. 2019; 17(8):438.Chicago/Turabian Style
Liu, Xiaoyang; Wang, Zixu; Yin, Fawen; Liu, Yuxin; Qin, Ningbo; Nakamura, Yoshimasa; Shahidi, Fereidoon; Yu, Chenxu; Zhou, Dayong; Zhu, Beiwei. 2019. "Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides." Mar. Drugs 17, no. 8: 438.
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