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Open AccessArticle

Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides

School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
National Engineering Research Center of Seafood, Dalian 116034, China
Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan
Department of Biochemistry, Memorial University of Newfoundland, St. John’s, NL A1B 3X9, Canada
Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, IA 50011, USA
Author to whom correspondence should be addressed.
Mar. Drugs 2019, 17(8), 438;
Received: 8 July 2019 / Revised: 23 July 2019 / Accepted: 24 July 2019 / Published: 25 July 2019
PDF [2029 KB, uploaded 6 August 2019]


In this study, three synthetic zinc-chelating peptides (ZCPs) derived from sea cucumber hydrolysates with limited or none of the common metal-chelating amino-acid residues were analyzed by flame atomic absorption spectroscopy, circular dichroism spectroscopy, size exclusion chromatography, zeta-potential, Fourier transform infrared spectroscopy, Raman spectroscopy and nuclear magnetic resonance spectroscopy. The amount of zinc bound to the ZCPs reached maximum values with ZCP:zinc at 1:1, and it was not further increased by additional zinc presence. The secondary structures of ZCPs were slightly altered, whereas no formation of multimers was observed. Furthermore, zinc increased the zeta-potential value by neutralizing the negatively charged residues. Only free carboxyl in C-terminus of ZCPs was identified as the primary binding site of zinc. These results provide the theoretical foundation to understand the mechanism of zinc chelation by peptides. View Full-Text
Keywords: zinc-chelating peptide; sea cucumber; chelation mechanism zinc-chelating peptide; sea cucumber; chelation mechanism

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Liu, X.; Wang, Z.; Yin, F.; Liu, Y.; Qin, N.; Nakamura, Y.; Shahidi, F.; Yu, C.; Zhou, D.; Zhu, B. Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides. Mar. Drugs 2019, 17, 438.

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