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Open AccessArticle

Quantitative Proteome Reveals Variation in the Condition Factor of Sea Urchin Strongylocentrotus nudus during the Fishing Season Using an iTRAQ-based Approach

1
School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
2
College of Food Science and Engineering, Hainan Tropical Ocean University, Hainan 572022, China
3
National Engineering Research Center of Seafood, Dalian 116034, China
*
Authors to whom correspondence should be addressed.
Mar. Drugs 2019, 17(7), 397; https://doi.org/10.3390/md17070397
Received: 31 May 2019 / Revised: 28 June 2019 / Accepted: 2 July 2019 / Published: 5 July 2019
To investigate the variation in the condition factor of the sea urchin Strongylocentrotus nudus (S. nudus), gonads were collected in May (MAY), June (JUN), and July (JUL), at the beginning (AUG-b) and end of August (AUG-e). Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) detection of the gonads revealed an obvious enhancement of the band at about 37 kDa from July, which was identified as transforming growth factor-beta-induced protein ig-h3 (TGFBI) by nanoLC-ESI-MS/MS. Gonadal proteins were identified by isobaric tagging for relative and absolute quantitation (iTRAQ), and regulation of the identified proteins in pairs of the collected groups was observed. A total of 174 differentially expressed proteins (DEPs) were identified. Seven of the DEPs showed significant correlations with both the gonad index (GI) and protein content. These correlations included 6-phosphogluconate dehydrogenase, decarboxylating isoform X2 (6PGD), CAD protein, myoferlin isoform X8, ribosomal protein L36 (RL36), isocitrate dehydrogenase [NADP], mitochondrial isoform X2 (IDH), multifunctional protein ADE2 isoform X3, sperm-activating peptides (SAPs) and aldehyde dehydrogenase, and mitochondrial (ALDH). However, TGFBI had no correlation with gonad index (GI) or protein content. 6PGD, IDH, multifunctional protein ADE2 isoform X3, and ALDH were shown to interact with each other and might play key roles in changing the condition factor of S. nudus gonads. View Full-Text
Keywords: Strongylocentrotus nudus; seasonal variation; gonad index; condition factor Strongylocentrotus nudus; seasonal variation; gonad index; condition factor
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Shang, W.-H.; Han, J.-R.; Yan, J.-N.; Du, Y.-N.; Xu, Y.-S.; Xue, C.-F.; Zhang, T.-T.; Wu, H.-T.; Zhu, B.-W. Quantitative Proteome Reveals Variation in the Condition Factor of Sea Urchin Strongylocentrotus nudus during the Fishing Season Using an iTRAQ-based Approach. Mar. Drugs 2019, 17, 397.

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